Non-targeted detection and differentiation of agonists versus antagonists, directly in bioprofiles of everyday products.

Analytica Chimica Acta
Ines KlingelhöferGertrud E Morlock

Abstract

Xenoestrogens exert antiandrogenic effects on the human androgen receptor. In the analytical field, such antagonists block the detection of testosterone and falsify results obtained by sum parameter assays. Currently, such agonistic versus antagonistic effects are not differentiated in complex mixtures. Oppositely acting hormonal effects present in products of everyday use can only be differentiated after tedious fractionation and isolation of the individual compounds along with subjection of each fraction/compound to the status quo bioassay testing. However, such long-lasting procedures are not suited for routine. Hence, we developed a fast bioanalytical tool that figures out agonists versus antagonists directly in complex mixtures. Exemplarily, 8 cosmetics and 15 thermal papers were analyzed. The determined antagonistic potentials of active compounds found were comparable to the ones of known antagonists (in reference shown for bisphenol A, 4-n-nonylphenol and four parabens). Relevant biological/chromatographic parameters such as cell viability, culture conditions, dose response curves, limits of biological detection/quantification and working range (shown for testosterone, dihydrotestosterone, nandrolone and trenbolone) were...Continue Reading

Citations

Mar 20, 2021·Analytica Chimica Acta·Gertrud E MorlockSabrina Brinkmann
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gertrud E MorlockMichel Dubourdeaux

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