Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1.

International Journal of Molecular Sciences
Olga TroitskayaOlga Koval

Abstract

The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX-7 mouse rhabdomyosarcoma cells with the hallmarks of immunogenic cell death (ICD): calreticulin and heat shock protein 70 (HSP70) externalization and high-mobility group box 1 protein (HMGB1) release. The intensity of HMGB1 release after the CAP treatment correlated directly with the basal extracellular HMGB1 level. Releasing from dying cells, HMGB1 can act as a proinflammatory cytokine. Our in vivo study demonstrated that cold atmospheric plasma induces a short-term two-times increase in serum HMGB1 level only in tumor-bearing mice with no effect in healthy mice. These findings support our hypothesis that CAP-dependent HMGB1 release from dying cancer cells can change the serum HMGB1 level. At the same time, we showed a weak cytokine response to CAP irradiation in healthy mice that can characterize CAP as an immune-safety physical antitumor approach.

References

Aug 10, 2002·Wiener medizinische Wochenschrift·P VaupelM Höckel
Dec 14, 2004·The Oncologist·Peter Vaupel, Louis Harrison
Jun 28, 2005·Trends in Immunology·Ingrid E DumitriuPatrizia Rovere-Querini
Dec 21, 2005·The Journal of Experimental Medicine·Noelia CasaresGuido Kroemer
Nov 6, 2007·Immunological Reviews·Marco E Bianchi, Angelo A Manfredi
Dec 22, 2007·Nature Reviews. Immunology·Laurence ZitvogelGuido Kroemer
Jun 14, 2008·Nature Reviews. Immunology·John A Hamilton
Jul 5, 2008·Arthritis Research & Therapy·David S PisetskyUlf Andersson
Mar 28, 2009·Science·Marco E Bianchi, Angelo A Manfredi
May 6, 2009·Journal of Leukocyte Biology·Lara CampanaPatrizia Rovere-Querini
Feb 4, 2010·Biochimica Et Biophysica Acta·Michal Stros
May 24, 2011·Cancer Research·Jitka FucikovaRadek Spísek
Jul 21, 2012·Science Translational Medicine·Laurie MengerGuido Kroemer
Mar 23, 2013·Frontiers in Immunology·Guanqiao LiMichael T Lotze
Jul 11, 2014·Molecular Aspects of Medicine·Rui KangDaolin Tang
Oct 3, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bhranti S ShahNadeen O Chahine
May 17, 2019·Anticancer Research·Yongdae YoonSeung Joon Baek
Nov 14, 2019·Acta Naturae·E A GolubitskayaO A Koval
Feb 8, 2020·Proceedings of the National Academy of Sciences of the United States of America·Guojun ChenZhen Gu
Mar 27, 2020·Journal for Immunotherapy of Cancer·Lorenzo GalluzziFrancesco M Marincola
Jun 21, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Olga TroitskayaOlga Koval

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Citations

Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Helena MotalnBoris Rogelj

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Methods Mentioned

BETA
flow cytometry
ELISA

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