Non-Thermal Plasma, as a New Physicochemical Source, to Induce Redox Imbalance and Subsequent Cell Death in Liver Cancer Cell Lines

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Barbora SmolkováAlexandr Dejneka

Abstract

Alteration of cancer cell redox status has been recognized as a promising therapeutic implication. In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Thus, the aim of this study was a rigorous biochemical analysis of interactions between NTP and liver cancer cells. The concept was validated using three different cell lines. We provide several distinct lines of evidence to support our findings; we use various methods (epifluorescent and confocal microscopy, clonogenic and cytotoxicity assays, Western blotting, pharmacological inhibition studies, etc.). We assessed the influence of NTP on three human liver cancer cell lines (Huh7, Alexander and HepG2). NTP treatment resulted in higher anti-proliferative effect against Alexander and Huh7 relative to HepG2. Our data clearly showed that the NTP-mediated alternation of mitochondrial membrane potential and dynamics led to ROS-mediated apoptosis in Huh7 and Alexander cells. Interestingly, plasma treatment resulted in p53 down-regulation in Huh7 cells. High levels of Bcl-2 protein express...Continue Reading

Citations

Feb 12, 2020·Macromolecular Bioscience·Ivana VíšováHana Vaisocherová-Lísalová
Oct 5, 2019·Cellular and Molecular Life Sciences : CMLS·Mariia LunovaOleg Lunov
Jul 25, 2019·Cancers·Sarmistha MitraNagendra Kumar Kaushik
Nov 7, 2019·Oxidative Medicine and Cellular Longevity·Angela Privat-MaldonadoSander Bekeschus
Sep 3, 2020·International Journal of Molecular Sciences·Barbora SmolkováOleg Lunov
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Hye Ran LeeChul-Ho Kim
Sep 28, 2021·ACS Applied Materials & Interfaces·Barbora SmolkováOleg Lunov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.