Abstract
Alteration of cancer cell redox status has been recognized as a promising therapeutic implication. In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Thus, the aim of this study was a rigorous biochemical analysis of interactions between NTP and liver cancer cells. The concept was validated using three different cell lines. We provide several distinct lines of evidence to support our findings; we use various methods (epifluorescent and confocal microscopy, clonogenic and cytotoxicity assays, Western blotting, pharmacological inhibition studies, etc.). We assessed the influence of NTP on three human liver cancer cell lines (Huh7, Alexander and HepG2). NTP treatment resulted in higher anti-proliferative effect against Alexander and Huh7 relative to HepG2. Our data clearly showed that the NTP-mediated alternation of mitochondrial membrane potential and dynamics led to ROS-mediated apoptosis in Huh7 and Alexander cells. Interestingly, plasma treatment resulted in p53 down-regulation in Huh7 cells. High levels of Bcl-2 protein express...Continue Reading
Citations
Nov 30, 2019·Cancers·Oleg LunovAlexandr Dejneka
Feb 12, 2020·Macromolecular Bioscience·Ivana VíšováHana Vaisocherová-Lísalová
Apr 25, 2020·Cells·Mariia UzhytchakOleg Lunov
Oct 5, 2019·Cellular and Molecular Life Sciences : CMLS·Mariia LunovaOleg Lunov
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Sep 28, 2021·ACS Applied Materials & Interfaces·Barbora SmolkováOleg Lunov