Non-thermal plasma induces immunogenic cell death in vivo in murine CT26 colorectal tumors

Oncoimmunology
Abraham LinVandana Miller

Abstract

Immunogenic cell death is characterized by the emission of danger signals that facilitate activation of an adaptive immune response against dead-cell antigens. In the case of cancer therapy, tumor cells undergoing immunogenic death promote cancer-specific immunity. Identification, characterization, and optimization of stimuli that induce immunogenic cancer cell death has tremendous potential to improve the outcomes of cancer therapy. In this study, we show that non-thermal, atmospheric pressure plasma can be operated to induce immunogenic cell death in an animal model of colorectal cancer. In vitro, plasma treatment of CT26 colorectal cancer cells induced the release of classic danger signals. Treated cells were used to create a whole-cell vaccine which elicited protective immunity in the CT26 tumor mouse model. Moreover, plasma treatment of subcutaneous tumors elicited emission of danger signals and recruitment of antigen presenting cells into tumors. An increase in T cell responses targeting the colorectal cancer-specific antigen guanylyl cyclase C (GUCY2C) were also observed. This study provides the first evidence that non-thermal plasma is a bone fide inducer of immunogenic cell death and highlights its potential for clinic...Continue Reading

References

Jan 16, 1998·Immunology and Cell Biology·J G Altin, E K Sloan
Feb 2, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H J Burstein
Feb 28, 2002·Annual Review of Immunology·Pierre GuermonprezSebastian Amigorena
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jason RiceFreda K Stevenson
Jan 28, 2003·Trends in Immunology·Antoni RibasJames S Economou
Mar 12, 2003·Journal of Leukocyte Biology·Andrea la SalaGiampiero Girolomoni
Dec 11, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Richard M GoldbergSteven R Alberts
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yu-Pei LiaoWilliam H McBride
Dec 21, 2005·The Journal of Experimental Medicine·Noelia CasaresGuido Kroemer
May 20, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kimberly E BeckJames C Yang
Jul 21, 2006·American Journal of Physiology. Cell Physiology·Charles W BellDavid S Pisetsky
Jul 22, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anthony D YangLee M Ellis
Dec 26, 2006·Nature Medicine·Michel ObeidGuido Kroemer
Feb 3, 2007·The EMBO Journal·Valeria V OrlovaTriantafyllos Chavakis
Sep 28, 2007·Clinical Pharmacology and Therapeutics·A E SnookS A Waldman
Nov 6, 2007·Immunological Reviews·Marco E Bianchi, Angelo A Manfredi
Dec 22, 2007·Nature Reviews. Immunology·Laurence ZitvogelGuido Kroemer
Jun 26, 2008·Journal of the National Cancer Institute·Adam E SnookScott A Waldman
Jan 24, 2009·The EMBO Journal·Theocharis PanaretakisGuido Kroemer
Apr 15, 2009·Nature Reviews. Immunology·Douglas R GreenGuido Kroemer
Dec 4, 2009·Clinical and Translational Science·Adam E SnookScott A Waldman
Jan 21, 2011·Cancer Metastasis Reviews·Oliver KeppGuido Kroemer
Aug 9, 2011·Biosensors & Bioelectronics·Jae Young KimSung-O Kim
Oct 8, 2011·British Journal of Cancer·M KeidarB Trink
Nov 1, 2011·Immunologic Research·Adam E SnookScott A Waldman
Dec 24, 2011·Nature·Ira MellmanGlenn Dranoff
Mar 23, 2012·Nature Reviews. Cancer·Matthew Vanneman, Glenn Dranoff
Nov 20, 2012·Annual Review of Immunology·Guido KroemerLaurence Zitvogel
Jan 22, 2013·Journal of Pediatric Surgery·Ryan M WalkAnthony D Sandler
May 18, 2013·Cell Death & Disease·O KryskoD V Krysko
Jul 16, 2013·Cell Death and Differentiation·I MartinsG Kroemer
Feb 7, 2014·International Journal of Cancer. Journal International Du Cancer·Jitka FucikovaRadek Spisek

❮ Previous
Next ❯

Citations

Apr 23, 2020·Antioxidants·Sander BekeschusMatthias B Stope
Jun 6, 2020·Nanotechnology·Xiaofeng DaiKostya Ken Ostrikov
Nov 7, 2019·Oxidative Medicine and Cellular Longevity·Angela Privat-MaldonadoSander Bekeschus
Apr 26, 2020·International Journal of Molecular Sciences·Dušan BranýHenrieta Škovierová
Feb 1, 2020·Oncoimmunology·Isaure VanmeerbeekAbhishek D Garg
Sep 3, 2020·International Journal of Molecular Sciences·Barbora SmolkováOleg Lunov
Jul 2, 2020·International Journal of Molecular Sciences·Jaehak LeeSeung Joon Baek
May 16, 2019·Applied Microbiology and Biotechnology·Juliana ŠimončicováBarbora Kaliňáková
Jul 24, 2020·International Journal of Molecular Sciences·Olga TroitskayaOlga Koval
Aug 25, 2020·Plasma Processes and Polymers·Hager MohamedVandana Miller
Aug 8, 2020·Nature Reviews. Clinical Oncology·Lorenzo GalluzziGuido Kroemer
Nov 19, 2020·Cancers·Xiaofeng DaiKostya Ken Ostrikov
Mar 27, 2021·British Journal of Cancer·Rajesh Kumar GandhirajanSander Bekeschus
May 1, 2021·International Journal of Molecular Sciences·Sander BekeschusRamona Clemen
May 1, 2021·Antioxidants·Eloisa SardellaPietro Favia
May 28, 2021·Cancer Biotherapy & Radiopharmaceuticals·Yanghua JieWeidong Chen
Jun 3, 2021·Vaccines·Ramona Clemen, Sander Bekeschus

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
FACS

Software Mentioned

ImageJ
ImmunoSpot

Related Concepts

Related Feeds

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.