Non-thermal plasma specifically kills oral squamous cell carcinoma cells in a catalytic Fe(II)-dependent manner

Journal of Clinical Biochemistry and Nutrition
Kotaro SatoShinya Toyokuni

Abstract

Oral cancer accounts for ~2% of all cancers worldwide, and therapeutic intervention is closely associated with quality of life. Here, we evaluated the effects of non-thermal plasma on oral squamous cell carcinoma cells with special reference to catalytic Fe(II). Non-thermal plasma exerted a specific killing effect on oral squamous cell carcinoma cells in comparison to fibroblasts. Furthermore, the effect was dependent on the amounts of catalytic Fe(II), present especially in lysosomes. After non-thermal plasma application, lipid peroxidation occurred and peroxides and mitochondrial superoxide were generated. Cancer cell death by non-thermal plasma was promoted dose-dependently by prior application of ferric ammonium citrate and prevented by desferrioxamine, suggesting the association of ferroptosis. Potential involvement of apoptosis was also observed with positive terminal deoxynucleaotidyl transferase-mediated dUTP nick end labeling and annexin V results. Non-thermal plasma exposure significantly suppressed the migratory, invasive and colony-forming abilities of squamous cell carcinoma cells. The oral cavity is easily observable; therefore, non-thermal plasma can be directly applied to the oral cavity to kill oral squamous ce...Continue Reading

References

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Citations

Jul 28, 2020·Cancers·Gabriella Pasqual-MeloSander Bekeschus
Jan 23, 2021·Archives of Biochemistry and Biophysics·Yasumasa OkazakiShinya Toyokuni
Feb 5, 2021·Signal Transduction and Targeted Therapy·Hong-Fa YanPeng Lei
May 9, 2021·Archives of Biochemistry and Biophysics·Yasumasa OkazakiShinya Toyokuni
Nov 10, 2020·Acta Pharmaceutica Sinica. B·Aqu AluXiawei Wei

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Methods Mentioned

BETA
flow cytometry
electrophoresis
confocal microscopy

Software Mentioned

ImageJ
GraphPad Prism

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