Non-thermal plasma treatment improves chicken sperm motility via the regulation of demethylation levels

Scientific Reports
Jiao Jiao ZhangDong Kee Jeong

Abstract

The quality of avian semen is an important economic trait in poultry production. The present study examines the in vitro effects of non-thermal dielectric barrier discharge plasma on chicken sperm to determine the plasma conditions that can produce the optimum sperm quality. Exposure to 11.7 kV of plasma for 20 s is found to produce maximum sperm motility by controlling the homeostasis of reactive oxygen species and boosting the release of adenosine triphosphate and respiratory enzyme activity in the mitochondria. However, prolonged exposure or further increase in plasma potential impairs the sperm quality in a time- and dose-dependent manner. Optimal plasma treatment of sperm results in upregulated mRNA and protein expression of antioxidant defense-related and energetic metabolism-related genes by increasing their demethylation levels. However, 27.6 kV of plasma exerts significant adverse effects. Thus, our findings indicate that appropriate plasma exposure conditions improve chicken sperm motility by regulating demethylation levels of genes involved in antioxidant defense and energetic metabolism.

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Citations

Aug 8, 2018·International Journal of Molecular Sciences·Jiao Jiao ZhangDong Kee Jeong
May 16, 2019·Applied Microbiology and Biotechnology·Juliana ŠimončicováBarbora Kaliňáková
Jul 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Mohsen GavahianCheorun Jo
Jun 3, 2021·International Journal of Molecular Sciences·Eva TvrdáEliška Gálová

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Methods Mentioned

BETA
MDA
methylation sequencing
Assay
PCR
protein assay
electrophoresis

Software Mentioned

CyMATE
Statistical Package for the Social Sciences ( SPSS
ImageJ

Related Concepts