PMID: 2120080Oct 1, 1990Paper

Non-tryptophan fluorescence and high molecular weight protein formation in lens crystallins of rats with chronic galactosemia: prevention by the aldose reductase inhibitor sorbinil

Experimental Eye Research
R H Nagaraj, Vincent M Monnier

Abstract

Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water soluble (P less than 0.001) and insoluble (P less than 0.005) lens fractions in galactosemic rats receiving the aldose reductase inhibitor sorbinil. High performance liquid chromatography of water soluble lens crystallins showed an increase in the content of high molecular weight proteins and the fluorescence associated with it. Sorbinil partly prevented the formation of such fluorescent high molecular weight proteins. Under reducing conditions, sodium dodecyl sulfate polyacrylamide gel electrophoresis of water soluble proteins revealed a distinct high molecular weight protein of 60 kDa in galactosemia. Sorbinil treatment completely abolished the formation of this protein. Western blotting using rabbit antiserum to bovine alpha-, beta- and gamma-crystallins revealed the presence of gamma, but not alpha- and beta-crystallins in the 60-kDa protein. Formation of this protein may result from trimerization of gamma-crystallin...Continue Reading

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Citations

May 1, 1991·Molecular Biology Reports·M A van Boekel
Jan 1, 1992·Pharmacology & Therapeutics·D R TomlinsonA L Carrington
Nov 24, 1998·Metabolism: Clinical and Experimental·K YanagisawaT Koike
Jan 1, 1995·European Journal of Pediatrics·G T Berry
Aug 1, 1992·Journal of Neuroscience Research·T Ebendal
Jul 12, 2003·Eye·M Donaldson, P M Dodson
Feb 18, 2004·Journal of Cataract and Refractive Surgery·José L MenezoRafael Martínez-Costa
Dec 1, 1994·International Journal of Peptide and Protein Research·S M Shaw, M J Crabbe
May 8, 2020·International Journal of Biological Macromolecules·Ejlal Mohamed AbdullahAjamaluddin Malik

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