Non-uniform cellular packing of the stratum corneum and permeability barrier function of intact skin: a high-resolution confocal laser scanning microscopy study using highly deformable vesicles (Transfersomes)

The British Journal of Dermatology
A Schätzlein, Gregor Cevc

Abstract

Novel, functional skin staining with fluorescent, ultradeformable lipid vesicles (Transfersomes, IDEA, Munich, Germany) was developed and combined with confocal laser scanning microscopy. This revealed the structural and barrier characteristics of intact skin to a resolution of > or = 0.2 micron, that is, to the limit of light microscopy. Different routes of penetration into the stratum corneum were visualized and new details in the skin anatomy and barrier were unveiled. Most prominent was the lateral inhomogeneity of the stratum corneum, where three to 10 neighbouring corneocyte 'columns' were found to form a cluster. Corneocyte edges inside each cluster intercalated extensively, but adjacent clusters were separated by 'gorges' a few micrometers deep; lipid packing was also less regular and tight in the intercluster region. Two quantitatively different hydrophilic pathways were found in the horny layer: an intercluster route with low penetration resistance comprising < or = 1% of the total or < or = 20% of the pathway area in the skin, and an intercorneocyte pathway that resists penetration better and is more abundant (> or = 3% of the skin or > or = 80% of the pathway area). This latter route is strongly tortuous, as it goes...Continue Reading

References

May 1, 1992·Pharmaceutical Research·R O Potts, R H Guy
Dec 1, 1991·The Journal of Investigative Dermatology·J A BouwstraW Bras
May 1, 1990·Journal of Microscopy·S J Jones, M L Taylor
Jul 1, 1989·The Journal of Investigative Dermatology·P W WertzD T Downing
Feb 1, 1989·The Journal of Investigative Dermatology·D C SwartzendruberD T Downing
Feb 1, 1988·The Journal of Investigative Dermatology·R R WarnerD A Taylor
Jun 1, 1987·The Journal of Investigative Dermatology·D C SwartzendruberD T Downing
Jun 1, 1974·The Journal of Investigative Dermatology·E ChristophersE B Laurence
Jul 26, 1995·Biochimica Et Biophysica Acta·S ZellmerJ Lasch
Jun 1, 1995·The Journal of Investigative Dermatology·M RajadhyakshaR R Anderson
Jan 1, 1994·Dermatology : International Journal for Clinical and Investigative Dermatology·L Bruckner-Tuderman
Jan 1, 1994·Dermatology : International Journal for Clinical and Investigative Dermatology·J A Veiro, P G Cummins
Jan 1, 1993·Methods in Cell Biology·L Majlof, P O Forsgren
Oct 1, 1995·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·G Gompper, D M Kroll
Aug 1, 1995·Physical Review. B, Condensed Matter·A O Caldeira, Castro Neto AH
Jul 2, 2005·Expert Opinion on Investigational Drugs·G Cevc
Jul 23, 2009·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Mariko Egawa, Tomoko Kajikawa

❮ Previous
Next ❯

Citations

Sep 1, 2005·Annals of Biomedical Engineering·Ana M Barbero, H F Frasch
Sep 11, 2007·Pharmaceutical Research·Nitin K SwarnakarN K Jain
Mar 17, 2004·Advanced Drug Delivery Reviews·Gregor Cevc
Mar 22, 2005·International Journal of Pharmaceutics·Prem N GuptaSuresh P Vyas
Jan 28, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·B Godin, E Touitou
May 28, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·A M de GraaffJ A Bouwstra
Jun 18, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·P Loan Honeywell-NguyenJoke A Bouwstra
Nov 7, 2003·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ramesh R BoinpallyBhaskara R Jasti
Aug 30, 2013·International Journal of Nanomedicine·Eder Lilia Romero, Maria Jose Morilla
May 13, 2003·Clinical Pharmacokinetics·Gregor Cevc
Jul 4, 2012·Advanced Drug Delivery Reviews·Dominik SelzerDirk Neumann
Jun 30, 2014·Colloids and Surfaces. B, Biointerfaces·Dolores C CarrerEder Lilia Romero
Nov 1, 2007·Expert Opinion on Drug Delivery·Vaibhav DubeyNarendra K Jain
Apr 5, 2013·Expert Opinion on Drug Delivery·Jun ChenBao-Chang Cai
Feb 6, 2008·Expert Opinion on Drug Delivery·Yu-Han Su, Jia-You Fang
Nov 2, 2006·Expert Opinion on Drug Delivery·Heather A E Benson
Jan 5, 2002·Journal of Receptor and Signal Transduction Research·J A BouwstraM Suhonen
Jun 27, 2012·Expert Opinion on Drug Delivery·Nina Dragicevic-Curic, Alfred Fahr
Jul 2, 2008·Expert Opinion on Drug Delivery·D CoscoD Paolino
Jul 9, 2013·Expert Opinion on Drug Metabolism & Toxicology·Sarah KüchlerWolfgang Friess
Jul 3, 2009·Expert Opinion on Drug Delivery·Chiara Sinico, Anna Maria Fadda
Nov 7, 2003·Journal of Pharmaceutical Sciences·H Frederick Frasch, Ana M Barbero
Oct 19, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Priya BathejaBozena Michniak-Kohn
Oct 13, 2009·International Journal of Pharmaceutics·Nina Dragicevic-CuricAlfred Fahr
Nov 3, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Gavish KumarAmla Chopra
Nov 1, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·B GeusensM Van Gele
Sep 2, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dolores C CarrerLuis A Bagatolli
Jun 10, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Johanna BenderMarica B Ericson
Jun 6, 2008·International Journal of Pharmaceutics·G CevcS Mazgareanu
May 24, 2008·International Journal of Pharmaceutics·Gregor CevcUlrich Vierl
Feb 19, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Nina Dragicevic-CuricAlfred Fahr
Jan 16, 2007·International Journal of Pharmaceutics·Mustafa M A ElsayedNawal M Khalafallah
Dec 13, 2006·International Journal of Pharmaceutics·J MontanariE L Romero
Jan 27, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gregor Cevc, Ulrich Vierl
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·R Alvarez-RománR H Guy
Oct 7, 2009·Biophysical Journal·Chinmay DasPeter D Olmsted
Nov 22, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·S Lombardi BorgiaM Schäfer-Korting
Apr 1, 2005·Drug Discovery Today. Technologies·P Loan Honeywell-Nguyen, Joke A Bouwstra
Oct 29, 2013·Journal of Liposome Research·Vipin Kumar SharmaBhaskar Mazumder
Mar 19, 2013·International Journal of Pharmaceutics·Valentina IannuccelliEliana Leo
Jun 15, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Mukul AshtikarJürgen Popp

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.