Non-uniform membrane probe distribution in resonance energy transfer: application to protein-lipid selectivity

Journal of Fluorescence
Ricardo C CapetaL M Loura

Abstract

Biological membranes are, at the molecular level, quasi-two dimensional systems. Membrane components are often distributed non-uniformly in the bilayer plane, as a consequence of lipid phase separation/domain formation or local enrichment/depletion of particular lipid species arising form favorable/unfavorable lipid-membrane protein interactions. Due to its explicit dependence on donor-acceptor distance or local acceptor concentration, resonance energy transfer (RET) has large potential in the characterization of membrane heterogeneity. RET formalisms for the basic geometric arrangements relevant for membranes have now been known for several decades. However, these formalisms usually assume uniform distributions, and more general models are required for the study of membrane lateral heterogeneity. We present a model that addresses the possibility of non-uniform acceptor (e.g., lipid probe) distribution around each donor (e.g., protein) in a membrane. It considers three regions with distinct local acceptor concentration, namely, an exclusion zone, the membrane bulk, and, lying in between, a region of enhanced probability of finding acceptors (annular region). Numerical solutions are presented, and convenient empirical fitting fu...Continue Reading

References

Nov 1, 1979·Biophysical Journal·P K Wolber, B S Hudson
Feb 26, 1987·Biochimica Et Biophysica Acta·C Gutierrez-MerinoA G Lee
Oct 2, 2002·European Biophysics Journal : EBJ·Ida C BoniniFrancisco J Barrantes
May 2, 2003·Nature·Youxing JiangRoderick MacKinnon

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Citations

Mar 20, 2010·European Biophysics Journal : EBJ·Luís M S LouraFábio Fernandes
Feb 23, 2012·Journal of Biomedical Optics·Evelyne DeplazesBen Corry
Nov 19, 2008·Biochimica Et Biophysica Acta·Luís M S LouraManuel Prieto
May 4, 2006·The Journal of Biological Chemistry·Maria L MolinaJose M González-Ros

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