Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

PloS One
Katharina DebowskiR Behr

Abstract

Groundbreaking studies showed that differentiated somatic cells of mouse and human origin could be reverted to a stable pluripotent state by the ectopic expression of only four proteins. The resulting pluripotent cells, called induced pluripotent stem (iPS) cells, could be an alternative to embryonic stem cells, which are under continuous ethical debate. Hence, iPS cell-derived functional cells such as neurons may become the key for an effective treatment of currently incurable degenerative diseases. However, besides the requirement of efficacy testing of the therapy also its long-term safety needs to be carefully evaluated in settings mirroring the clinical situation in an optimal way. In this context, we chose the long-lived common marmoset monkey (Callithrix jacchus) as a non-human primate species to generate iPS cells. The marmoset monkey is frequently used in biomedical research and is gaining more and more preclinical relevance due to the increasing number of disease models. Here, we describe, to our knowledge, the first-time generation of marmoset monkey iPS cells from postnatal skin fibroblasts by non-viral means. We used the transposon-based, fully reversible piggyback system. We cloned the marmoset monkey reprogrammin...Continue Reading

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Citations

Dec 15, 2015·Stem Cells International·Bentolhoda FereydouniRüdiger Behr
Dec 3, 2016·Cell Biology and Toxicology·Dharmendra KumarWilfried A Kues
Jun 22, 2017·Stem Cells and Development·Scott C VermilyeaThaddeus G Golos
Jun 17, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Lena WalentaArtur Mayerhofer
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May 18, 2019·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Toshiaki WatanabeErika Sasaki
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Mar 20, 2020·Stem Cells and Development·Seiji ShiozawaHideyuki Okano
Aug 28, 2020·World Journal of Stem Cells·Dharmendra KumarWilfried A Kues

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Datasets Mentioned

BETA
KM279352
KM279353
GSE64966

Methods Mentioned

BETA
transfection
PCR
SMA
RNA-Seq
PCRs
performed
biopsy

Software Mentioned

package goseq
htseq
R
CASAVA
DESeq
goseq
PyroMark Q24
Bioconductor
ENSEMBL
BaseCaller

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