Non-viral suicide gene therapy in cervical, oral and pharyngeal carcinoma cells with CMV- and EEV-plasmids

The Journal of Gene Medicine
Nejat DüzgüneşKrystyna Konopka

Abstract

Cervical cancer is the third most common cause of cancer in women. The 5-year survival rate in oropharyngeal squamous cell carcinomas is approximately 50% and this rate has not improved in recent decades. These cancers are accessible to direct intervention. We examined the ability of a highly efficient non-viral vector, TransfeX (ATCC, Manassas, VA, USA), to deliver the suicide gene HSV-tk to cervical, oral and pharyngeal cancer cells and to induce cytotoxicity following the administration of the prodrug, ganciclovir. HeLa cervical carcinoma, HSC-3 and H357 oral squamous cell carcinoma and FaDu pharyngeal carcinoma cells were transfected with cytomegalovirus (CMV)- or enhanced episomal vector (EEV)-driven HSV-tk plasmids and treated with ganciclovir for 24-120 h. Cell viability was assessed by Alamar blue. The viability of HeLa cells was reduced to only 30-40%, despite the very high levels of transgene expression. By contrast, the viability of HSC-3 cells was reduced to 10%, although transgene expression was 18-fold lower than that in HeLa cells. An approximately five-fold higher transgene expression was obtained with the EEV-plasmid than from the CMV-plasmid. Nevertheless, HeLa cell viability after suicide gene + ganciclovir t...Continue Reading

References

Jul 1, 1996·The Annals of Otology, Rhinology, and Laryngology·E A GoebelJ A Kern
Feb 17, 2000·Biochimica Et Biophysica Acta·C Tros de Ilarduya, N Düzgüneş
Aug 30, 2000·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·S C Shin, J Y Kim
Apr 20, 2001·Advanced Drug Delivery Reviews·M C Pedroso de LimaN Düzgüneş
Dec 19, 2002·Biochimica Et Biophysica Acta·H FanecaM C Pedrosa de Lima
Apr 14, 2004·The Journal of Gene Medicine·Marika RuponenArto Urtti
Nov 20, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gabi U DachsAdam V Patterson
Oct 11, 2014·World Journal of Clinical Oncology·Shaniqua L McGraw, Jeanne M Ferrante
Jan 1, 2014·Molecular Therapy. Methods & Clinical Development·Patrick F FordeDeclan M Soden
Jul 29, 2015·CA: a Cancer Journal for Clinicians·Angela C ChiBrad W Neville
Oct 30, 2016·Therapeutic Delivery·Jennifer CheungNejat Düzgünes

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Citations

Jun 5, 2019·Pharmaceutics·Nejat Düzgüneş, Krystyna Konopka
Apr 17, 2021·Frontiers in Immunology·Qijie ZhaoZhangang Xiao

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