Non-viral transfer of BDNF and uPA stimulates peripheral nerve regeneration

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
M KaragyaurV Tkachuk

Abstract

Peripheral nerves connect brain and spinal cord with the extremities and inner organs, and nerves injury can lead the disability and social exclusion. Growth factors and other natural stimulators of regeneration processes look very promising as future medicines. In our study, we tested the influence of genetic constructions that contain genes of brain-derived neurotrophic factor and urokinase plasminogen activator on nerve's structure and function after traumatic and ischemic injuries. Injection of pVax1-hBDNF and pVax1-muPA after traumatic injury led to better restoration of nerve's structure and function compared to similar parameters of control group mice. In ischemic injury model pVax1-hBDNF and pVax1-muPA slowed and reduced the damage progression and stimulated nerve regeneration as well. However, the treatment with pVax1-muPA was less effective after the traumatic injury. As we chose a non-viral method of gene delivery during our study the optimal conditions of plasmid intramuscular delivery were also determined.

References

May 5, 1999·Journal of Anatomy·G Terenghi
Mar 10, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hyung-Suk JangDuk-Kyung Kim
Jun 4, 2004·Neurosurgical Focus·Mark G Burnett, Eric L Zager
Mar 19, 2005·The Journal of Bone and Joint Surgery. British Volume·R K KadiyalaM D Cassell
Sep 6, 2005·Bulletin of Experimental Biology and Medicine·O P BalezinaS M Strukova
Sep 20, 2005·Journal of Clinical Laboratory Analysis·Fabian RohnerRichard F Hurrell
Jul 27, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dmitry O TraktuevYelena V Parfyonova
Feb 2, 2012·Neurobiology of Disease·Eunsil ChoJoo-Yong Lee

❮ Previous
Next ❯

Citations

May 24, 2018·Nanomedicine·Chen AijieShao Longquan
Jan 1, 2020·The Journal of Pharmacology and Experimental Therapeutics·Maxim KaragyaurVsevolod Tkachuk
May 9, 2019·PeerJ·Sebastiaan Van De VijverMichele Giugliano
Feb 17, 2019·International Journal of Molecular Sciences·Peter P NimiritskyPavel I Makarevich
May 26, 2018·Bulletin of Experimental Biology and Medicine·E S PetrovaD E Korzhevskii
May 22, 2021·Tissue Engineering. Part C, Methods·Maxim KaragyaurDmitry Stambolsky
Mar 9, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·M А BoldyrevaYe V Parfyonova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.