Noncoding RNAs: Possible Players in the Development of Fluorosis

BioMed Research International
Atul P DaiwileKrishnamurthi Kannan

Abstract

Fluorosis is caused by excess of fluoride intake over a long period of time. Aberrant change in the Runt-related transcription factor 2 (RUNX2) mediated signaling cascade is one of the decisive steps during the pathogenesis of fluorosis. Up to date, role of fluoride on the epigenetic alterations is not studied. In the present study, global expression profiling of short noncoding RNAs, in particular miRNAs and snoRNAs, was carried out in sodium fluoride (NaF) treated human osteosarcoma (HOS) cells to understand their possible role in the development of fluorosis. qPCR and in silico hybridization revealed that miR-124 and miR-155 can be directly involved in the transcriptional regulation of Runt-related transcription factor 2 (RUNX2) and receptor activator of nuclear factor κ-B ligand (RANKL) genes. Compared to control, C/D box analysis revealed marked elevation in the number of UG dinucleotides and D-box sequences in NaF exposed HOS cells. Herein, we report miR-124 and miR-155 as the new possible players involved in the development of fluorosis. We show that the alterations in UG dinucleotides and D-box sequences of snoRNAs could be due to NaF exposure.

References

Oct 16, 2002·Annals of the Rheumatic Diseases·D Holstead JonesJ M Penninger
Aug 6, 2004·Endocrine Reviews·Terence M DohertyTripathi B Rajavashisth
Jan 1, 2005·Environmental Health Perspectives·Serap Erdal, Susan N Buchanan
Sep 26, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Xiang-Hang LuoEr-Yuan Liao
Sep 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhaoyong LiGary S Stein
Jun 11, 2009·The Journal of Biological Chemistry·Xiaojuan SunRichard M Terek
Sep 1, 2009·Bone·Charles A O'Brien
Oct 16, 2009·Journal of Dental Research·J G CarvalhoM A R Buzalaf
Jun 22, 2010·RNA·Kersten T SchroederDavid M J Lilley
Sep 18, 2010·Nucleic Acids Research·Markus BrameierJens Gruber
Jul 28, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Manoj NairNick Ovsenek
Sep 29, 2011·Current Osteoporosis Reports·Anna Teti
Dec 27, 2011·Nucleic Acids Research·Michelle S ScottAngus I Lamond
Apr 24, 2012·Leukemia·W ValleronP Brousset
Apr 9, 2013·Indian Journal of Endocrinology and Metabolism·Gilberto Paz-FilhoJulio Licinio
Apr 23, 2013·BioMed Research International·Grigoriy A StepanovVladimir A Richter
Jun 6, 2013·International Journal of Endocrinology·Aurora PattiRanuccio Nuti
Oct 15, 2013·Nature·Audrone LapinaiteTeresa Carlomagno
Feb 4, 2014·Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry·Soumyadeep SainT Chakrabarti
Jul 31, 2014·Nucleic Acids Research·Gabrielle Deschamps-FrancoeurMichelle S Scott

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE57550

Methods Mentioned

BETA
PCR
deamination

Software Mentioned

miRANDA
Expression Consoles
Expression Console
GeneSpring GX
Affymetrix Expression Console

Related Concepts

Related Feeds

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.