Noncovalent Interactions between Trimethylamine N-Oxide (TMAO), Urea, and Water

The Journal of Physical Chemistry. B
Sarah G ZetterholmNathan I Hammer

Abstract

Trimethylamine N-oxide (TMAO) and urea are two important osmolytes with their main significance to the biophysical field being in how they uniquely interact with proteins. Urea is a strong protein destabilizing agent, whereas TMAO is known to counteract urea's deleterious effects. The exact mechanisms by which TMAO stabilizes and urea destabilizes folded proteins continue to be debated in the literature. Although recent evidence has suggested that urea binds directly to amino acid side chains to make protein folding less thermodynamically favored, it has also been suggested that urea acts indirectly to denature proteins by destabilizing the surrounding hydrogen bonding water networks. Here, we elucidate the molecular level mechanism of TMAO's unique ability to counteract urea's destabilizing nature by comparing Raman spectroscopic frequency shifts to the results of electronic structure calculations of microsolvated molecular clusters. Experimental and computational data suggest that the addition of TMAO into an aqueous solution of urea induces blue shifts in urea's H-N-H symmetric bending modes, which is evidence for direct interactions between the two cosolvents.

References

Dec 1, 1990·Hypertension·A Garcia-Perez, M B Burg
Sep 24, 1982·Science·P H YanceyG N Somero
Jul 18, 2001·Journal of the American Chemical Society·M IkeguchiK Shimizu
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian J Bennion, Valerie Daggett
May 14, 2003·Biochemistry·Youxing Qu, D Wayne Bolen
Feb 20, 2004·Journal of the American Chemical Society·Joseph D BatchelorGary J Pielak
Apr 13, 2006·Journal of the American Chemical Society·Maeng-Eun Lee, Nico F A van der Vegt
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Timothy O StreetGeorge D Rose
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Y L A Rezus, H J Bakker
Feb 3, 2007·The Journal of Physical Chemistry. B·Yoshihito HayashiAkio Yasuda
Mar 22, 2007·Journal of the American Chemical Society·Sandip Paul, Grenfell N Patey
Jun 22, 2007·The Journal of Physical Chemistry. B·Sandip Paul, G N Patey
Sep 14, 2007·The Journal of Physical Chemistry. B·Jose Manuel Hermida-RamónGunnar Karlström
Sep 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew AutonD Wayne Bolen
Jun 4, 2008·Annual Review of Biochemistry·D Wayne Bolen, George D Rose
Aug 8, 2008·The Journal of Physical Chemistry. B·Sandip Paul, G N Patey
Oct 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lan HuaB J Berne
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Peter J Rossky
Jan 7, 2009·Journal of the American Chemical Society·Ronen ZangiB J Berne
Nov 4, 2009·Biophysical Journal·Filip MeersmanMichel H J Koch
Jan 9, 2010·Annual Review of Physical Chemistry·Yanjie Zhang, Paul S Cremer
Feb 4, 2010·Journal of the American Chemical Society·Deepak R CanchiAngel E García
Mar 18, 2010·The Journal of Physical Chemistry. B·A IdrissiG Vergoten
Jun 10, 2010·The Journal of Physical Chemistry. a·Austin A HowardNathan I Hammer
Jan 22, 2011·Journal of the American Chemical Society·Hironori KokuboB Montgomery Pettitt
May 24, 2011·The Journal of Physical Chemistry. B·Katherine L MunroeNathan I Hammer
Jul 2, 2011·Physical Chemistry Chemical Physics : PCCP·Filip MeersmanMichel H J Koch
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Emily J GuinnM Thomas Record
Oct 5, 2011·Journal of the American Chemical Society·Laura B SaglePaul S Cremer
Oct 14, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·J Coleman HowardGregory S Tschumper
Nov 11, 2011·The Journal of Chemical Physics·Rahul Sarma, Sandip Paul
Jan 28, 2012·Journal of the American Chemical Society·Jörg Rösgen, Ruby Jackson-Atogi
Sep 14, 2012·The Journal of Physical Chemistry. B·Deepak R CanchiAngel E Garcia
Jan 10, 2013·Annual Review of Physical Chemistry·Deepak R Canchi, Angel E García
May 18, 2013·The Journal of Physical Chemistry. a·Ashley M WrightNathan I Hammer
Jun 27, 2013·The Journal of Physical Chemistry. B·Jagannath MondalB J Berne
Dec 18, 2013·The Journal of Physical Chemistry. B·Beate Moeser, Dominik Horinek
Dec 20, 2013·The Journal of Physical Chemistry. B·Kristina A CuellarNathan I Hammer
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jianqiang MaFeng Gai

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Citations

May 15, 2020·Physical Chemistry Chemical Physics : PCCP·Christoph J SahleChristian Sternemann
May 3, 2019·The Journal of Physical Chemistry. B·Ewa Anna Oprzeska-Zingrebe, Jens Smiatek
Jun 5, 2020·The Journal of Physical Chemistry. B·Pritam GangulyJoan-Emma Shea
Sep 7, 2021·The Journal of Physical Chemistry. B·Archita Maiti, Snehasis Daschakraborty

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