Noncovalent labeling of proteins in capillary electrophoresis with laser-induced fluorescence detection

Cell Biochemistry and Biophysics
C L Colyer

Abstract

Interest in the use of capillary electrophoresis (CE) as a tool for protein separations continues to grow. Additionally, laser-induced fluorescence (LIF) detection schemes promise ultrasensitive detection of small quantities of these important biomolecules following their separation. In most cases, LIF detection of proteins necessitates their prior derivatization with a fluorescent label molecule. To minimize the amount of additional sample handling and time associated with such labeling procedures, not to mention the sometimes-stringent pH and temperature controls they require, noncovalent labeling is presented as a viable alternative. This review article considers established methods for noncovalent labeling of proteins for their subsequent analysis by CE-LIF. Label molecules suitable for excitation and emission in the ultraviolet, visible, and near-infrared regions of the spectrum are enumerated for a variety of protein analytes.

Citations

Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Heli Sirén, Anne Karttunen
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Frank WelderChrista L Colyer
Sep 19, 2007·Analytical Chemistry·Shuqing WuShaorong Liu
Nov 6, 2004·Nature Biotechnology·Arthur J Chirino, Anthony Mire-Sluis
Feb 13, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kengo KanazakiHideo Saji
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Eva Szöko, Tamás Tábi
Dec 1, 2006·Electrophoresis·Ana M García-CampañaHuguette Fabre
Jan 19, 2008·Electrophoresis·Kimberly D ChichesterChrista L Colyer
Aug 30, 2002·Analytical Chemistry·Rezik A AgbariaIsiah M Warner

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