Nov 1, 1977

Noncytotoxic IgE-mediated release of histamine and serotonin from murine mastocytoma cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
J D TaurogH Metzger


Cultured murine mastocytoma (AB-CBF1-MCT-1) cells were stimulated to release endogenous or incorporated histamine or serotonin by an IgE-mediated mechanisms without loss of viability. Stimulation was achieved by incubation of the cells with rat IgE-anti-IgE, rat IgE-anti-light chain, fluoresceinated rat IgE-anti-fluorescein, IgE-enriched mouse anti-ovalbumin-ovalbumin, or covalently linked dimers of rat IgE, at doses similar to those optimal for normal peritoneal mast cells. Active cell metabolism and Ca++ were required to obtain release. Despite the latter, no dose of the calcium ionophore, A23187, could be found which caused release without concomitant cytotoxicity. Phosphatidylserine did not enhance release.

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Mentioned in this Paper

Histamine Measurement
Metabolic Process, Cellular
Anti-Immunoglobulin E antibody
Calcium Ionophores
Mast Cell
Serotonin Measurement

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