Nondestructive Assessment of Engineered Cartilage Composition by Near Infrared Spectroscopy

Annals of Biomedical Engineering
Cushla M McGoverinNancy Pleshko

Abstract

Tissue engineering presents a strategy to overcome the limitations of current tissue healing methods. Scaffolds, cells, external growth factors and mechanical input are combined in an effort to obtain constructs with properties that mimic native tissues. However, engineered constructs developed using similar culture environments can have very different matrix composition and biomechanical properties. Accordingly, a nondestructive technique to assess constructs during development such that appropriate compositional endpoints can be defined is desirable. Near infrared spectroscopy (NIRS) analysis is a modality being investigated to address the challenges associated with current evaluation techniques, which includes nondestructive compositional assessment. In the present study, cartilage tissue constructs were grown using chondrocytes seeded onto polyglycolic acid (PGA) scaffolds in similar environments in three separate tissue culture experiments and monitored using NIRS. Multivariate partial least squares (PLS) analysis models of NIR spectra were calculated and used to predict tissue composition, with biochemical assay information used as the reference data. Results showed that for combined data from all tissue culture experimen...Continue Reading

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Citations

May 24, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xinhong WangYuhai Guo
Jul 29, 2017·Applied Spectroscopy·Mithilesh PrakashJuha Töyräs
Jul 31, 2018·Annals of the New York Academy of Sciences·James P KarchnerNancy Pleshko
Nov 4, 2016·Annals of Biomedical Engineering·Jaakko K SarinIsaac O Afara
Jan 21, 2020·Journal of Biophotonics·No'ad ShanasNancy Pleshko
Mar 7, 2020·Tissue Engineering. Part C, Methods·Shital KandelNancy Pleshko
Sep 29, 2019·Scientific Reports·Matt SponheimerSahra Talamo
Sep 29, 2017·The Analyst·William QueridoNancy Pleshko
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·William QueridoNancy Pleshko

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