Nondestructive Characterization of Stem Cell Neurogenesis by a Magneto-Plasmonic Nanomaterial-Based Exosomal miRNA Detection

ACS Nano
Jin-Ho LeeKi-Bum Lee

Abstract

The full realization of stem cell-based treatments for neurodegenerative diseases requires precise control and characterization of stem cell fate. Herein, we report a multifunctional magneto-plasmonic nanorod (NR)-based detection platform to address the limitations associated with the current destructive characterization methods of stem cell neurogenesis. Exosomes and their inner contents have been discovered to play critical roles in cell-cell interactions and intrinsic cellular regulations and have received wide attention as next-generation biomarkers. Moreover, exosomal microRNAs (miRNA) also offer an essential avenue for nondestructive molecular analyses of cell cytoplasm components. To this end, our developed nondestructive, selective, and sensitive detection platform has (i) an immunomagnetic active component for exosome isolation and (ii) a plasmonic/metal-enhanced fluorescence component for sensitive exosomal miRNA detection to characterize stem cell differentiation. In a proof-of-concept demonstration, our multifunctional magneto-plasmonic NR successfully detected the expression level of miRNA-124 and characterized neurogenesis of human-induced pluripotent stem cell-derived neural stem cells in a nondestructive and eff...Continue Reading

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Citations

Feb 20, 2020·Sensors·Jin-Ha ChoiJeong-Woo Choi
Feb 25, 2020·Nanoscale·Jin-Ho LeeJeong-Woo Choi
Aug 23, 2020·Materials·Jin-Ha ChoiJeong-Woo Choi
Nov 26, 2020·Biosensors·Jinho YoonJeong-Woo Choi
Dec 31, 2020·Biomedicines·Intan Rosalina SuhitoTae-Hyung Kim
Feb 27, 2021·Analytical Chemistry·He YanYong Zeng
Sep 1, 2021·Advanced Science·Li MinShutao Wang

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