Nondestructive evaluation of progressive neuronal changes in organotypic rat hippocampal slice cultures using ultrahigh-resolution optical coherence microscopy

Neurophotonics
Fengqiang LiChao Zhou

Abstract

Three-dimensional tissue cultures have been used as effective models for studying different diseases, including epilepsy. High-throughput, nondestructive techniques are essential for rapid assessment of disease-related processes, such as progressive cell death. An ultrahigh-resolution optical coherence microscopy (UHR-OCM) system with [Formula: see text] axial resolution and [Formula: see text] transverse resolution was developed to evaluate seizure-induced neuronal injury in organotypic rat hippocampal cultures. The capability of UHR-OCM to visualize cells in neural tissue was confirmed by comparison of UHR-OCM images with confocal immunostained images of the same cultures. In order to evaluate the progression of neuronal injury, UHR-OCM images were obtained from cultures on 7, 14, 21, and 28 days in vitro (DIVs). In comparison to DIV 7, statistically significant reductions in three-dimensional cell count and culture thickness from UHR-OCM images were observed on subsequent time points. In cultures treated with kynurenic acid, significantly less reduction in cell count and culture thickness was observed compared to the control specimens. These results demonstrate the capability of UHR-OCM to perform rapid, label-free, and nond...Continue Reading

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Citations

Nov 8, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Akiyuki HasegawaTatsuya Shimizu
Oct 1, 2015·Optics Letters·Eunjung MinWoonggyu Jung
Apr 30, 2016·Optics Letters·Caroline MagnainDavid A Boas
May 14, 2016·Optics Letters·Hui WangDavid Boas
Oct 11, 2016·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Jing MenChao Zhou
Apr 23, 2019·Tissue Engineering. Part C, Methods·Kristy DerrPaige Derr

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