Abstract
Upon contact with a wet surface, mature pollen grains hydrate and release proteins including allergens. Knowledge of the release mechanism of allergens that are mainly localized intracellularly may allow the design of strategies for inhibition of allergen release and the consequent sensitization process. An improved pollen chromatography was performed with Artemisia vulgaris and Lilium longiflorum pollen. Using three elution media of different pH, osmolality and salt concentration mimicking various types of wet surfaces, the time-dependent elution profiles of total protein, a cell wall-bound acid phosphatase activity (acPase), allergenic (profilin, Art v 1) and nonallergenic molecules (14-3-3 protein, actin) were monitored. The release kinetics of total protein and cell wall-bound acPase followed an exponential decrease in both pollen species indicating a diffusion-based protein release, whereas the elution profiles of profilin, Art v 1 and 14-3-3 protein showed nondiffusion characteristics. No general dependence on pH, osmolality or salt concentration of the elution media was observable in the elution profiles. Under the applied conditions, actin was not released indicating that the pollen grains remained intact during the elu...Continue Reading
References
Jun 1, 1988·The Journal of Allergy and Clinical Immunology·J N BaraniukC E Buckley
Jan 1, 1993·International Archives of Allergy and Immunology·S VrtalaR Valenta
May 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M GroteR Valenta
Apr 16, 1998·Allergy·N W de JongH de Groot
Jun 23, 1998·The Plant Cell·B C GibbonC J Staiger
Feb 2, 1999·International Archives of Allergy and Immunology·M Grote
Feb 27, 1999·Current Opinion in Plant Biology·L K Wilhelmi, D Preuss
Aug 27, 1999·Trends in Plant Science·H J ChungR J Ferl
Feb 25, 2000·International Archives of Allergy and Immunology·A Bufe
Aug 22, 2001·Molecular Biology of the Cell·L VidaliP K Hepler
Aug 29, 2001·Planta·H PertlG Obermeyer
Oct 26, 2001·Current Opinion in Immunology·H Behrendt, W M Becker
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P K HeplerA Y Cheung
Jul 9, 2002·Protoplasma·Hala IbrahimGerhard Obermeyer
Aug 10, 2002·Allergy·J CarnésM Blanca
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin HimlyFátima Ferreira
Jan 18, 2003·Biological Chemistry·Nicole WopfnerFatima Ferreira
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J H CroweL M Crowe
Citations
Dec 14, 2011·Plant Cell Reports·Marc SiegertGerhard Obermeyer
Jan 8, 2014·BMC Plant Biology·Xiao-Guang WangNian-Jun Teng
Apr 12, 2005·Plant Biology·H PertlG Obermeyer
Mar 30, 2020·Fungal Biology·Łukasz GrewlingMatt Smith
Apr 14, 2021·Environmental Research·Chiara SuannoStefano Del Duca
Jan 21, 2020·The Science of the Total Environment·Łukasz GrewlingAgata Frątczak