Nondiffusional release of allergens from pollen grains of Artemisia vulgaris and Lilium longiflorum depends mainly on the type of the allergen

International Archives of Allergy and Immunology
Claudia HoidnGerhard Obermeyer

Abstract

Upon contact with a wet surface, mature pollen grains hydrate and release proteins including allergens. Knowledge of the release mechanism of allergens that are mainly localized intracellularly may allow the design of strategies for inhibition of allergen release and the consequent sensitization process. An improved pollen chromatography was performed with Artemisia vulgaris and Lilium longiflorum pollen. Using three elution media of different pH, osmolality and salt concentration mimicking various types of wet surfaces, the time-dependent elution profiles of total protein, a cell wall-bound acid phosphatase activity (acPase), allergenic (profilin, Art v 1) and nonallergenic molecules (14-3-3 protein, actin) were monitored. The release kinetics of total protein and cell wall-bound acPase followed an exponential decrease in both pollen species indicating a diffusion-based protein release, whereas the elution profiles of profilin, Art v 1 and 14-3-3 protein showed nondiffusion characteristics. No general dependence on pH, osmolality or salt concentration of the elution media was observable in the elution profiles. Under the applied conditions, actin was not released indicating that the pollen grains remained intact during the elu...Continue Reading

References

Jun 1, 1988·The Journal of Allergy and Clinical Immunology·J N BaraniukC E Buckley
Jan 1, 1993·International Archives of Allergy and Immunology·S VrtalaR Valenta
May 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M GroteR Valenta
Apr 16, 1998·Allergy·N W de JongH de Groot
Jun 23, 1998·The Plant Cell·B C GibbonC J Staiger
Feb 2, 1999·International Archives of Allergy and Immunology·M Grote
Feb 27, 1999·Current Opinion in Plant Biology·L K Wilhelmi, D Preuss
Aug 27, 1999·Trends in Plant Science·H J ChungR J Ferl
Feb 25, 2000·International Archives of Allergy and Immunology·A Bufe
Aug 22, 2001·Molecular Biology of the Cell·L VidaliP K Hepler
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P K HeplerA Y Cheung
Dec 12, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Martin HimlyFátima Ferreira
Jan 18, 2003·Biological Chemistry·Nicole WopfnerFatima Ferreira
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J H CroweL M Crowe

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.