Nonencapsulated Streptococcus pneumoniae Cause Acute Otitis Media in the Chinchilla That Is Enhanced by Pneumococcal Surface Protein K

Open Forum Infectious Diseases
L E KellerL S McDaniel

Abstract

Use of the pneumococcal conjugate vaccine has led to serotype replacement of carriage and acute otitis media (AOM) pneumococcal isolates. Increases in nonencapsulated Streptococcus pneumoniae (NESp) isolates have also occurred, and there are increasing reports of NESp-associated disease. Disease prevalence and virulence factors of NESp isolates have not been studied. A chinchilla model of pneumococcal AOM was utilized, and disease was assessed through bacterial enumeration along with scoring visible signs of pathology. An adhesion-invasion assay using a human epithelial cell line was performed. Nonencapsulated Streptococcus pneumoniae strains containing pneumococcal surface protein K (PspK) were more likely to cause AOM and pathology upon infection. Deletion of PspK from an isolate significantly reduced bacterial loads. Increased epithelial cell adhesion correlated with increased virulence of NESp isolates naturally lacking PspK. Furthermore, expression of PspK by an avirulent NESp resulted in virulence. The presence of PspK increased the disease potential of NESp. Pneumococcal surface protein K is not the only virulence factor of NESp in AOM. Expression of PspK in an avirulent NESp mediated the progression to pneumococcal dise...Continue Reading

References

Dec 1, 1986·Journal of Bacteriology·J YotherD E Briles
Jan 18, 1995·JAMA : the Journal of the American Medical Association·L F McCaig, J M Hughes
Aug 21, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R GonzalesM A Sande
Mar 21, 2003·The New England Journal of Medicine·Michael MartinCynthia G Whitney
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Mar 5, 2004·The Lancet Infectious Diseases·D BogaertP W M Hermans
Jan 18, 2005·Lancet·Kim A BrogdenChristopher E Taylor
Jan 27, 2006·Microbiology·Simone Bergmann, Sven Hammerschmidt
Jun 17, 2006·Nature Reviews. Microbiology·John L TelfordGuido Grandi
Apr 26, 2007·FEMS Immunology and Medical Microbiology·Chinwendu OnwubikoLarry S McDaniel
Aug 30, 2008·Critical Reviews in Microbiology·Gowrisankar RajamEdwin W Ades
Apr 28, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R Sá-LeãoH de Lencastre
Sep 15, 2009·Lancet·Katherine L O'BrienUNKNOWN Hib and Pneumococcal Global Burden of Disease Study Team
Jun 30, 1931·The Journal of Experimental Medicine·O T Avery, R Dubos
Apr 21, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Terhi TapiainenMatti Uhari
Nov 30, 2010·International Journal of Medical Microbiology : IJMM·Bernd KreikemeyerAndreas Podbielski
Jan 22, 2011·Diagnostic Microbiology and Infectious Disease·Qingfu XuMichael E Pichichero
Jan 29, 2011·Science·Nicholas J CroucherStephen D Bentley
Feb 11, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A VainioA Virolainen
Feb 27, 2013·Pediatrics·Allan S LieberthalDavid E Tunkel
Jul 28, 2013·Genome Announcements·Lance E KellerD Ashley Robinson
Feb 11, 2014·Nature Genetics·Claire ChewapreechaStephen D Bentley

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Citations

Dec 27, 2015·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Muneki HotomiNoboru Yamanaka
May 5, 2016·IDCases·Cheshil DixitLarry S McDaniel
Sep 17, 2016·Pneumonia·Anukul T Shenoy, Carlos J Orihuela
Apr 5, 2017·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Stephen J BarenkampW Edward Swords
Feb 3, 2018·The Journal of Infectious Diseases·Haley R PipkinsLarry S McDaniel
Mar 24, 2016·MBio·Lance E KellerLarry S McDaniel
May 26, 2016·MBio·Larry S McDaniel, Edwin Swiatlo
Aug 30, 2019·PLoS Pathogens·Jessica L Bradshaw, Larry S McDaniel
Jul 27, 2017·Frontiers in Cellular and Infection Microbiology·Guangchun Bai, Jorge E Vidal
Jun 27, 2019·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Noriko TakeuchiNaruhiko Ishiwada
Mar 7, 2021·Vaccines·Julio SempereFernando González-Camacho

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BETA
flow cytometry

Software Mentioned

Prism
GraphPad

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