Nonendosomal cellular uptake of ligand-free, positively charged gold nanoparticles

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Ulrike TaylorDetlef Rath

Abstract

Gold nanoparticles (GNPs) have interesting optical properties, such as exceptionally high quantum yields and virtually limitless photostability. Therefore, they show the potential for applications as biomarkers especially suitable for in vivo and long-term studies. The generation of GNPs using pulsed laser light rather than chemical means provides nanoparticles, which are remarkably stable in a variety of media without the need of stabilizing agents or ligands. This stabilization is achieved by partial oxidation of the gold surface resulting in positively charged GNPs. However, little is known about cellular uptake of such ligand-free nanoparticles, their intracellular fate, or cell viability after nanoparticle contact. The current work is aimed to explore the response of a bovine cell line to GNP exposure mainly using laser scanning confocal microscopy (LSCM) supported by other techniques. Cultured bovine immortalized cells (GM7373) were coincubated with GNP (average diameter 15 nm, 50 microM Au) for 2, 24, and 48 h. The detection of GNP-associated light scattering by the LSCM facilitated a clear distinction between GNP-containing cells and the negative controls. After 48 h, 75% of cells had visibly incorporated nanoparticles....Continue Reading

References

Mar 1, 1983·Journal of Cellular Physiology·J B GrinspanE M Levine
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·S SchultzD A Schultz
Feb 24, 2001·International Archives of Occupational and Environmental Health·G Oberdörster
Dec 3, 2002·Nature Biotechnology·Jyoti K JaiswalSanford M Simon
May 31, 2003·Science·Daniel R LarsonWatt W Webb
Sep 24, 2004·Microbiology and Immunology·Amane ShioharaKenji Yamamoto
Oct 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haifeng WangJi-Xin Cheng
Oct 27, 2005·Journal of Cell Science·Juan M Falcón-PérezEsteban C Dell'Angelica
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ravi ShuklaMurali Sastry
Apr 13, 2006·Nano Letters·B Devika ChithraniWarren C W Chan
Jun 19, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Hirak K PatraAnjan Kr Dasgupta
Oct 30, 2007·Small·Yu PanWilli Jahnen-Dechent
Jan 1, 2008·Small·Nastassja LewinskiRebekah Drezek

❮ Previous
Next ❯

Citations

Jan 30, 2013·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·B JeremicN Filipovic
Mar 6, 2012·Chemical Society Reviews·Rochelle R ArvizoPriyabrata Mukherjee
Oct 22, 2011·Journal of Nanobiotechnology·María Carolina DuránHugo Murua Escobar
Nov 13, 2012·International Journal of Nanomedicine·Eleonore Fröhlich
Aug 15, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R M ZuckerW K Boyes
Sep 1, 2010·Reproduction in Domestic Animals = Zuchthygiene·W A KuesH Niemann
Jun 22, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R M ZuckerW K Boyes
Jan 22, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Natalia BarkalinaKevin Coward
Jul 5, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lei SunZhiwei Sun
Jul 16, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Dandan ChenLeshuai W Zhang
Mar 1, 2016·Scientific Reports·Elena BossiGiovanni Bernardini
Sep 14, 2017·Scientific Reports·Daniele ZanellaGiovanni Bernardini
Mar 31, 2015·Beilstein Journal of Nanotechnology·Ulrike TaylorDetlef Rath
Jan 1, 2019·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Tae Joon ChoVincent A Hackley
Nov 30, 2019·Nanotechnology·Vladimir IvoševSandrine Lacombe
Apr 19, 2020·Biochimica Et Biophysica Acta. Biomembranes·Matthew J SydorAndrij Holian

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis