Nonenzymatic chromophore attachment in biliproteins: conformational control by the detergent Triton X-100

Biochimica Et Biophysica Acta
Kai-Hong ZhaoHugo Scheer

Abstract

While chromophore attachment to alpha-subunits of cyanobacterial biliproteins has been studied in some detail, little is known about this process in beta-subunits. The ones of phycoerythrocyanin and C-phycocyanin each carry two phycocyanobilin (PCB) chromophores covalently attached to cysteins beta84 and beta155. The differential nonenzymatic reconstitution of PCB to the apoproteins, PecA, PecB, CpcA and CpcB, as well as to mutant proteins of the beta-subunits lacking either one of the two binding cysteins, was studied using overexpression of the respective genes. PCB adds selectively to Cys-84 of CpcA, CpcB, PecA, and PecB, but the bound chromophore has a nonnative configuration, and in the case of CpcA, is partly oxidized to mesobiliverdin (MBV). The oxidation is independent of thiols but can be suppressed by ascorbate. The addition to Cys-beta84 is suppressed in the presence of detergents like Triton X-100, in favor of an addition to Cys-beta155 yielding the correctly bound chromophore. Triton X-100 also inhibits oxidation of the chromophore during addition to CpcA. The effect of Triton X-100 was studied on the isolated components of the reconstitution system. Absorption, fluorescence and circular dichroism spectra indicate ...Continue Reading

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Citations

Oct 4, 2007·Photosynthesis Research·Jun-Ming TuHugo Scheer
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kai-Hong ZhaoHugo Scheer
Oct 30, 2009·The Journal of Biological Chemistry·Michaela KupkaKai-Hong Zhao
Sep 30, 2010·The Journal of Biological Chemistry·Jessica WiethausNicole Frankenberg-Dinkel
Jul 5, 2012·Molecular Microbiology·Xiang GaoYu-Zhong Zhang
Feb 21, 2008·Molecular Microbiology·H Scheer, K-H Zhao
Jun 28, 2007·The Journal of Biological Chemistry·Stephan BöhmKai-Hong Zhao
Sep 11, 2009·The FEBS Journal·David FrommholzAnnette E Kaiser
Mar 28, 2009·Journal of the American Chemical Society·Jun-Ming TuKai-Hong Zhao
Dec 29, 2004·Biochimica Et Biophysica Acta·Kai-Hong ZhaoHugo Scheer
May 2, 2006·Journal of Photochemistry and Photobiology. B, Biology·Baosheng GeYuhong Ren

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