Abstract
While chromophore attachment to alpha-subunits of cyanobacterial biliproteins has been studied in some detail, little is known about this process in beta-subunits. The ones of phycoerythrocyanin and C-phycocyanin each carry two phycocyanobilin (PCB) chromophores covalently attached to cysteins beta84 and beta155. The differential nonenzymatic reconstitution of PCB to the apoproteins, PecA, PecB, CpcA and CpcB, as well as to mutant proteins of the beta-subunits lacking either one of the two binding cysteins, was studied using overexpression of the respective genes. PCB adds selectively to Cys-84 of CpcA, CpcB, PecA, and PecB, but the bound chromophore has a nonnative configuration, and in the case of CpcA, is partly oxidized to mesobiliverdin (MBV). The oxidation is independent of thiols but can be suppressed by ascorbate. The addition to Cys-beta84 is suppressed in the presence of detergents like Triton X-100, in favor of an addition to Cys-beta155 yielding the correctly bound chromophore. Triton X-100 also inhibits oxidation of the chromophore during addition to CpcA. The effect of Triton X-100 was studied on the isolated components of the reconstitution system. Absorption, fluorescence and circular dichroism spectra indicate ...Continue Reading
References
Feb 1, 1977·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H ScheerC Krauss
May 7, 1976·Analytical Biochemistry·M M Bradford
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C D FairchildA N Glazer
Feb 5, 1991·Journal of Molecular Biology·M DuerringR Huber
Feb 5, 1990·Journal of Molecular Biology·M DuerringH Zuber
Apr 20, 1986·Journal of Molecular Biology·T SchirmerM L Hackert
Aug 5, 1987·Journal of Molecular Biology·T SchirmerR Huber
Jul 1, 1986·Analytical Biochemistry·T R Berkelman, J C Lagarias
Aug 15, 1970·Nature·U K Laemmli
May 26, 1995·The Journal of Biological Chemistry·L J JungA N Glazer
Jun 2, 1995·Journal of Molecular Biology·K BrejcS Steinbacher
Apr 1, 1995·Biophysical Journal·A A Demidov, M Mimuro
Nov 15, 1993·European Journal of Biochemistry·R Ficner, R Huber
Oct 11, 1996·Journal of Molecular Biology·W R ChangD C Liang
Jun 30, 1996·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Jun 30, 1996·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·W ReuterM E Than
Mar 10, 2000·FEBS Letters·K H ZhaoH Scheer
May 20, 2000·Photochemistry and Photobiology·H FoerstendorfF Siebert
Aug 19, 2000·Plant Molecular Biology·R D WillowsS I Beale
Nov 7, 2000·Biochemistry·S H Wu, J C Lagarias
Apr 3, 2001·The Plant Cell·N FrankenbergJ C Lagarias
May 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·X Q WangD C Liang
Jul 21, 2001·Biophysical Journal·T JiangD C Liang
Oct 10, 2001·Biochemistry·M StorfK H Zhao
Jan 5, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Sep 17, 2002·European Journal of Biochemistry·Kai-Hong ZhaoHugo Scheer
Sep 21, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yasukazu NakamuraSatoshi Tabata
Oct 18, 2002·European Journal of Biochemistry·Georg WiegandWolfgang Reuter
Jan 27, 2004·Biochimica Et Biophysica Acta·Peter ZehetmayerAndreas Zumbusch
Mar 24, 2004·Biochemistry·Tilman LamparterJoaquin Abian
Citations
Jan 29, 2008·Biotechnology Letters·Yu YangSong Qin
Oct 4, 2007·Photosynthesis Research·Jun-Ming TuHugo Scheer
Nov 21, 2007·Applied Biochemistry and Biotechnology·Xiangyu GuanXuexi Tang
Aug 30, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kai-Hong ZhaoHugo Scheer
Oct 30, 2009·The Journal of Biological Chemistry·Michaela KupkaKai-Hong Zhao
Sep 30, 2010·The Journal of Biological Chemistry·Jessica WiethausNicole Frankenberg-Dinkel
Jan 18, 2008·The Journal of Biological Chemistry·Nicolle A SaunéeWendy M Schluchter
Feb 3, 2009·The Journal of Biological Chemistry·Nicolas BlotKai-Hong Zhao
Mar 17, 2010·Applied and Environmental Microbiology·Avijit BiswasWendy M Schluchter
Mar 19, 2013·PloS One·Veronika Anyigoh AtemnkengAnnette Kaiser
Jan 22, 2014·Applied Microbiology and Biotechnology·Dalu DongPing Yu
Jul 5, 2012·Molecular Microbiology·Xiang GaoYu-Zhong Zhang
Feb 21, 2008·Molecular Microbiology·H Scheer, K-H Zhao
Mar 8, 2006·The FEBS Journal·Kai-Hong ZhaoHugo Scheer
Apr 29, 2006·The Journal of Biological Chemistry·Gaozhong ShenDonald A Bryant
Feb 3, 2006·The Journal of Biological Chemistry·Kai-Hong ZhaoHugo Scheer
Jun 28, 2007·The Journal of Biological Chemistry·Stephan BöhmKai-Hong Zhao
Sep 11, 2009·The FEBS Journal·David FrommholzAnnette E Kaiser
Sep 27, 2007·The Journal of Biological Chemistry·Kai-Hong ZhaoMing Zhou
Mar 28, 2009·Journal of the American Chemical Society·Jun-Ming TuKai-Hong Zhao
Dec 29, 2004·Biochimica Et Biophysica Acta·Kai-Hong ZhaoHugo Scheer
May 2, 2006·Journal of Photochemistry and Photobiology. B, Biology·Baosheng GeYuhong Ren