PMID: 6405389May 1, 1983Paper

Nonenzymatic glucosylation of homologous low density lipoprotein and albumin renders them immunogenic in the guinea pig

Proceedings of the National Academy of Sciences of the United States of America
J L WitztumA Kesaniemi

Abstract

Nonenzymatic glucosylation of low density lipoprotein (LDL) and other plasma and structural proteins is enhanced in diabetics. Because conjugated carbohydrates are known to play an important role in the immunogenicity of proteins, we sought to determine if glucosylation of LDL (yielding Glc-LDL) and albumin would make them immunogenic. Therefore, we immunized guinea pigs with homologous glucosylated proteins and measured antibody response by solid-phase radioimmunoassay. Glucosylation of LDL in the presence of cyanoborohydride yields glucitol-lysine as the glucose adduct. Immunization with this Glc-LDL yielded a high-titered antiserum that reacted specifically with guinea pig Glc-LDL but not native LDL. Glucitol-lysine was an effective competitor for binding to the antibody, as were other reductively glucosylated human proteins. Glucosylation of LDL by incubation with glucose in the absence of a reducing agent yields fructosyllysine as the glucose adduct. This product, which has been demonstrated in human plasma, was also immunogenic, though the antiserum produced was of lower titer and affinity. Homologous glucosylated albumin was also immunogenic. These data suggest that nonenzymatic glucosylation of proteins could lead to au...Continue Reading

Citations

Oct 1, 1994·Diabetes/metabolism Reviews·G H Tomkin, D Owens
Jan 1, 1990·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·N KossovskyC V Deshpandey
Nov 16, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y OheK Shima
Jan 1, 1991·Progress in Lipid Research·C L MalmendierC Delcroix
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L WitztumM Fisher
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W PalinskiJ L Witztum
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·W PalinskiJ L Witztum
Sep 13, 2011·The Journal of Biological Chemistry·Daniel Steinberg
Aug 18, 2005·Cardiology in Review·Tanaz Kermani, William H Frishman
Jul 9, 2008·Current Opinion in Lipidology·Nahla YounisPaul N Durrington
Jul 1, 1991·Diabetic Medicine : a Journal of the British Diabetic Association·M H Dominiczak
Aug 1, 1997·International Journal of Cardiology·R W Wissler, P D Group
Oct 21, 2011·Thrombosis and Haemostasis·S C A de Jager, J Kuiper
May 15, 2013·American Journal of Hematology·Pamela GhoshJose A Halperin
Aug 1, 1994·Human Pathology·J D SeidmanL E Phillips
Jan 1, 1987·Annals of the New York Academy of Sciences·J E O'MullaneE Tomlinson
Jul 1, 1992·Human Pathology·J E TomaszewskiV A Livolsi
Mar 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S G YoungS Bernstein
Nov 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·H R SlaterJ Shepherd
Mar 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A N OrekhovV N Smirnov
Oct 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·W PalinskiJ L Witztum
May 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·M E RosenfeldJ L Witztum
Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·S Ylä-HerttualaJ L Witztum
May 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·W PalinskiJ L Witztum
Mar 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·M FukumotoY Nishizawa
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P van der SluijsD K Meijer
Dec 1, 1988·Diabetic Medicine : a Journal of the British Diabetic Association·S G GilbeyD Vergani
Feb 3, 2005·Clinical Chemistry·Eugene O ApostolovAlexei G Basnakian
Apr 5, 1990·The New England Journal of Medicine·T J Merimee
May 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·W PalinskiJ L Witztum
Apr 6, 1989·The New England Journal of Medicine·D SteinbergJ L Witztum

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