Nonenzymatic glycosylation of human serum albumin and its effect on antibodies profile in patients with diabetes mellitus.

PloS One
Alok RaghavKhursheed Alam

Abstract

Albumin glycation and subsequent formation of advanced glycation end products (AGEs) correlate with diabetes and associated complications. Human Serum Albumin (HSA) was modified with D-glucose for a 40 day period under sterile conditions at 37°C. Modified samples along with native HSA (unmodified) were analyzed for structural modifications by UV and fluorescence, FTIR, Liquid chromatography mass spectrometry (LCMS) and X-ray crystallography. New-Zealand white female rabbits immunized with AGEs, represent auto-antibodies formation as assessed by competitive and direct binding enzyme-linked immunosorbent assay (ELISA). Neo-epitopesagainst In-vitro formed AGEs were characterized in patients with diabetes mellitus type 2 (n = 50), type 1 (n = 50), gestational diabetes (n = 50) and type 2 with chronic kidney disease (CKD) with eGFR level 60-89 mL/min (n = 50) from serum direct binding ELISA. Glycated-HSA showed amarked increase in hyperchromicity of 65.82%,71.98%, 73.62% and 76.63% at λ280 nm along with anincreasein fluorescence intensity of 65.82%, 71.98%, 73.62% and 76.63% in glycated-HSA compared to native. FTIR results showed theshifting of Amide I peak from 1656 cm_1 to 1659 cm_1 and Amide II peak from 1554 cm_1 to 1564 cm_1 in...Continue Reading

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Citations

Dec 21, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agnieszka SzkudlarekMałgorzata Maciążek-Jurczyk
Apr 2, 2019·Mediators of Inflammation·Benjamin Wilde, Antonios Katsounas
Jun 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Namita KumariNavakanta Bhat
Aug 10, 2021·Frontiers in Chemistry·Dylan Nicholas TabangLingjun Li
Feb 11, 2020·Current Protein & Peptide Science·Afreen KhanamMohd Aslam Yusuf

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Methods Mentioned

BETA
enzyme-linked
ELISA
x-ray diffraction
fluorescence
X-ray

Software Mentioned

stat graphics

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