PMID: 1303150Sep 1, 1992Paper

Nonenzymatic incorporation of glucose and galactose into brain cytoskeletal proteins in vitro

Neurochemistry International
M R BrownH R Knull

Abstract

Initial studies demonstrated the loss of lysine and simultaneous appearance of glucitollysine in intracellular proteins following incubation with sugar. For example, when a crude nervous tissue cytoskeletal preparation was incubated in 100 mM glucose for 10 days, > 60% of the lysine residues were modified. Over 20% of the lysyl residues in a spinal cord neurofilament preparation are susceptible to Schiff base formation after one day and over 30% following five days of incubation with 100 mM glucose. When incubated with 100 mM galactose, F- and G-actin were found to be significantly modified in as few as 15 h, with > 70% of the lysyl residues lost. After 45 h of incubation, > 90% of the residues had been modified. These data also indicate that many of the lysyl residues in F- and G-actin are exposed and very susceptible to modification by sugar. This rapid and extensive modification of lysine in actin in vitro suggest that it may be modified in diabetic nervous tissue.

References

Apr 1, 1979·Metabolism: Clinical and Experimental·A CeramiV M Monnier
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·V J StevensA Cerami
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C E GuthrowJ W Baynes
Jun 1, 1976·Journal of Neurochemistry·J D Pardee, J R Bamburg
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D N Haney, H F Bunn
Mar 1, 1975·Acta paediatrica Scandinavica·J DitzelN D Peters
Nov 1, 1985·The Journal of Clinical Investigation·A D BaronJ M Olefsky
May 16, 1988·European Journal of Biochemistry·L MillerE Reisler
Oct 25, 1974·Brain Research·H R KnullW W Wells
Jun 1, 1970·The Journal of Clinical Investigation·H F Bunn, R W Briehl
Aug 22, 1969·Biochemical and Biophysical Research Communications·S RahbarH M Ranney
Aug 1, 1980·The Journal of Clinical Investigation·J Jakobsen, P Sidenius
Sep 1, 1980·Diabetologia·P Sidenius, J Jakobsen
Sep 30, 1982·Biochemical and Biophysical Research Communications·J SasakiG L Cottam
Feb 1, 1983·The Biochemical Journal·C S Tsai, J H White
Jan 1, 1984·Annals of Neurology·M J Brown, A K Asbury
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L WitztumM Fisher
Jan 1, 1982·Neuroscience·S TsukitaH Ishikawa
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S K WilliamsM W Bitensky
Jan 1, 1981·The Journal of Cell Biology·S TsukitaM Kurokawa

❮ Previous
Next ❯

Citations

Jan 3, 2016·The American Journal of Pathology·Warren G Tourtellotte
Dec 1, 2012·Current Opinion in Cell Biology·Jonathan R Terman, Anna Kashina
Jan 27, 2015·Neuroscience Letters·Christopher R Cashman, Ahmet Höke
Jul 22, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·J ZhangG X Shen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.