Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR

Chembiochem : a European Journal of Chemical Biology
Aaron E EngelhartNicholas V Hud

Abstract

Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.

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Citations

Jan 31, 2013·Nucleosides, Nucleotides & Nucleic Acids·Heike VogelClemens Richert
Jan 24, 2014·Angewandte Chemie·Charles N BirtsAli Tavassoli
Apr 28, 2016·Bioorganic & Medicinal Chemistry Letters·Michael J CatalanoKent S Gates
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Nov 10, 2018·Organic & Biomolecular Chemistry·Alejandro Gimenez MolinaMichael Smietana
Jan 1, 2015·Chemical Science·Renaud BarbeyronMichael Smietana
Mar 3, 2021·ELife·Alexandra KühnleinDieter Braun
Mar 8, 2018·Organic Letters·Tommi ÖsterlundPasi Virta
Aug 24, 2017·Biochemistry·John M BeierleLuke J Leman

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