PMID: 6295177Jan 1, 1983Paper

Nonenzymatic separation of myocardial cell nuclei from whole heart tissue

The American Journal of Physiology
C C LiewM J Sole


A simple and rapid nonenzymatic method has been developed to isolate myocardial cell nuclei from whole heart tissue. This method consists of a controlled disruption of cells followed by isopycnic gradient centrifugation. We have reviewed and compared our method to others more lengthy and laborious. By using a number of criteria, such as morphometric measurements, chemical composition, functional studies, specific nuclear protein markers, and mathematical analysis, we show that the nonenzymatic digestion method provides a most useful technique for the study of the biochemistry of the myocardial cell nucleus.


Oct 1, 1976·Journal of Molecular and Cellular Cardiology·M Masson-PévetJ De Bruijne
Mar 1, 1977·Archives of Biochemistry and Biophysics·B B FarmerH R Besch
May 1, 1977·Journal of Molecular and Cellular Cardiology·A F CutillettaR Zak
Jun 1, 1970·Circulation Research·M N BerryJ Scheuer
Feb 1, 1973·The Biochemical Journal·F Gonzalez-Mujica, A P Mathias
Sep 1, 1974·Analytical Biochemistry·M R GlickW J Reddy
Jan 1, 1974·Advances in Enzyme Regulation·A G Gornall, C C Liew
Aug 1, 1968·The Biochemical Journal·I R JohnstonD Ridge
Nov 1, 1970·Comparative Biochemistry and Physiology·S Bloom
Mar 1, 1969·Archives of Biochemistry and Biophysics·S Panyim, R Chalkley
Sep 1, 1980·Archives of Biochemistry and Biophysics·R L KaoE H Williams
May 1, 1980·Nature·J Pehrson, R D Cole
May 15, 1980·The Biochemical Journal·G Jackowski, C C Liew
Jul 1, 1980·Cell Biology International Reports·C C LiewG E Morris
Jun 3, 1960·Science·I HARARY, B FARLEY


Sep 1, 1992·Medical & Biological Engineering & Computing·R H MitchellJ M Anderson
May 1, 1985·Archives internationales de physiologie et de biochimie·J C Henquin
Mar 1, 1987·Veterinary Pathology·C A Picut, R M Lewis
Aug 1, 1993·Journal of Cardiovascular Electrophysiology·A V Panfilov, J P Keener
Jun 5, 1998·The American Journal of Physiology·F HaddadK M Baldwin
Jul 18, 2001·Current Opinion in Nephrology and Hypertension·J J Grantham
Mar 1, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Sara DanziIrwin Klein
Apr 1, 2004·European Journal of Human Genetics : EJHG·Hyunho KimSeongman Kang
Oct 23, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Agnes Kenessey, Kaie Ojamaa
Jan 5, 2006·Canadian Journal of Physiology and Pharmacology·Wim J LammersBetty Stephen
Jul 1, 2008·The Journal of Membrane Biology·P F OliveiraH G Ferreira
Jul 16, 2014·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jared J Grantham
May 21, 2014·Current Opinion in Nephrology and Hypertension·Christine DeislDaniel G Fuster
Mar 1, 1988·Pacing and Clinical Electrophysiology : PACE·G F TomaselliJ C Griffin
Jan 1, 1986·Annals of the New York Academy of Sciences·P Ronner
Oct 1, 1987·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·E D AvnerG H Fettermann
Apr 15, 1988·Hospital Practice·C Thompson
Oct 15, 1984·Experientia·W J MalaisseA Sener
Nov 1, 1996·Annals of Biomedical Engineering·S WangF A Roberge
Jan 1, 1987·Veterinary Research Communications·C A Picut, R M Lewis
Jul 29, 1993·The New England Journal of Medicine·M Ye, J J Grantham
May 26, 2001·Circulation Research·D Camper-KirbyM A Sussman
Oct 19, 2001·Anesthesiology·M KainumaT Kanno
Dec 13, 2005·BMC Endocrine Disorders·Subhadra C GunawardanaDavid W Piston
Jun 15, 2004·BMC Endocrine Disorders·Subhadra C GunawardanaDavid W Piston
Jan 1, 1990·Annals of the New York Academy of Sciences·J P Keener
Apr 1, 1988·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·E D AvnerD Ellis
Feb 15, 1987·Experientia·S Weidmann
Jul 21, 1999·Journal of Cardiovascular Electrophysiology·J Wu, D P Zipes
Jan 1, 1991·Journal of Mathematical Biology·J P Keener
Sep 2, 1998·The American Journal of Physiology·B D ShamesX Meng
Sep 14, 1999·The American Journal of Physiology·C E HowlettH Fliss
Sep 6, 2002·Renal Failure·Konstantinos MavromatidisGeorge Tsapas
Sep 24, 2004·The Anatomical Record. Part B, the New Anatomist·Robert H AndersonAntoon F M Moorman
Nov 1, 1996·Annals of Biomedical Engineering·F A RobergeL J Leon
Jul 25, 2015·Digestive Diseases and Sciences·Sandeep B SubramanyaWim J Lammers
Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·G CandianoG M Ghiggeri
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·D A TerrerosF E Cuppage
Apr 6, 2006·Circulation·Francisco J PérezChristine M Schubert
Feb 1, 1986·Digestive Diseases and Sciences·T J McGarrityD A Rasbach
Oct 21, 2000·Journal of Molecular and Cellular Cardiology·D AwaisF Ismail-Beigi

Related Concepts

Ascending Aorta Structure
Cell Fractionation
Cell Nucleus
Cell Separation
Centrifugation, Density Gradient
Centrifugation, Isopycnic
Collagenase-Like Peptidase
Closure by Clamp
DNA, Double-Stranded

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