Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1 F0 -ATP synthase

Proceedings of the National Academy of Sciences of the United States of America
Víctor G Almendro-VediaIván López-Montero

Abstract

ATP synthase is a rotating membrane protein that synthesizes ATP through proton-pumping activity across the membrane. To unveil the mechanical impact of this molecular active pump on the bending properties of its lipid environment, we have functionally reconstituted the ATP synthase in giant unilamellar vesicles and tracked the membrane fluctuations by means of flickering spectroscopy. We find that ATP synthase rotates at a frequency of about 20 Hz, promoting large nonequilibrium deformations at discrete hot spots in lipid vesicles and thus inducing an overall membrane softening. The enhanced nonequilibrium fluctuations are compatible with an accumulation of active proteins at highly curved membrane sites through a curvature-protein coupling mechanism that supports the emergence of collective effects of rotating ATP synthases in lipid membranes.

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Citations

Mar 16, 2018·Journal of Physics. Condensed Matter : an Institute of Physics Journal·T V Sachin KrishnanShigeyuki Komura
Nov 9, 2019·Physical Review Letters·Naomi OppenheimerMichael J Shelley
Jun 27, 2019·Communications Biology·Elena Beltrán-HerediaFrancisco Monroy
Jun 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nika MarušičRumiana Dimova
Jan 12, 2020·Communications Biology·Elena Beltrán-HerediaFrancisco Monroy
Apr 14, 2021·Scientific Reports·Hiroshi Noguchi, Olivier Pierre-Louis
May 31, 2021·Archives of Biochemistry and Biophysics·Víctor Almendro-VediaIván López-Montero
May 27, 2020·The Journal of Physical Chemistry. a·Kisung LeeSteve Granick
Aug 19, 2021·Nature Communications·Lado OtrinTanja Vidaković-Koch

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Methods Mentioned

BETA
confocal microscopy
ion exchange chromatography

Software Mentioned

MATLAB
ImageJ
Nikon NIS - Elements

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