Nonergodic and conformational control of the electron capture dissociation of protein cations

Proceedings of the National Academy of Sciences of the United States of America
Kathrin BreukerFred W McLafferty

Abstract

Electron capture dissociation (ECD) MS is proving to be unusually valuable for "top down" protein sequencing and identification/localization of posttranslational modifications, because the ECD product ions can represent cleavages between most of a protein's amino acids. As proposed, this unusual reactivity results from immediate local utilization, before randomization, of much of the relatively large ( approximately 6 eV) energy from the electron reaction with the multiply charged protein ion, minimizing the effect of differences in the backbone bond dissociation energies. However, others conclude that e(-) capture produces a labile free radical species for which backbone cleavage is the lowest energy reaction. Supporting the nonergodic mechanism, ECD of ubiquitin (M + 12H)(12+) ions also yields thermalized radical (M + 12H)(11+.) ions that instead lose H. when activated. Also, the ECD spectrum of ubiquitin (M + 13H)(13+) ions is nearly unchanged by heating from 25 degrees C to 125 degrees C, demonstrating that this increase in thermal energy is small compared to the energy driving the reaction. These results support initial capture of the electron in a long-lived high-n Rydberg state, followed by internal conversion to the pro...Continue Reading

References

Apr 11, 2000·Journal of the American Society for Mass Spectrometry·D M HornF W McLafferty
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Siu Kwan SzeFred W McLafferty
May 30, 2002·Journal of the American Chemical Society·Kathrin BreukerFred W McLafferty
Jun 12, 2002·Journal of the American Society for Mass Spectrometry·Ethan R BadmanDavid E Clemmer
Jul 18, 2002·Journal of the American Chemical Society·Jeremiah A Nummela, Barry K Carpenter
Nov 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·HanBin OhFred W McLafferty
Nov 26, 2002·FEMS Microbiology Letters·Akira YanoNobuhiro Hanada
Mar 13, 2003·Journal of the American Chemical Society·Frantisek Turecek, Erik A Syrstad
Jul 17, 2003·Journal of the American Chemical Society·Nancy LeymariePeter B O'Connor
Dec 6, 2003·Science·Christian TannerSamuel Leutwyler
Jun 17, 2004·Journal of the American Chemical Society·Motoya KohtaniMartin F Jarrold
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·John E P SykaDonald F Hunt

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Citations

Jun 24, 2006·Biotechnology Letters·Ray Bakhtiar, Ziqiang Guan
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Fuyu GuanJeffrey Rudy
Apr 6, 2012·Journal of the American Society for Mass Spectrometry·Owen S SkinnerKathrin Breuker
Apr 25, 2012·Journal of the American Society for Mass Spectrometry·Roman A ZubarevMikhail M Savitski
Sep 6, 2012·Journal of the American Society for Mass Spectrometry·Tawnya G Flick, Evan R Williams
Apr 4, 2013·Journal of the American Society for Mass Spectrometry·Owen S SkinnerFred W McLafferty
Apr 10, 2013·Journal of the American Society for Mass Spectrometry·Marija MentinovaScott A McLuckey
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Erik A Syrstad, Frantisek Turecek
Jun 28, 2005·Journal of the American Society for Mass Spectrometry·Weidong CuiJames P Reilly
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Ivan K Chu, Corey N W Lam
Oct 29, 2005·Journal of the American Society for Mass Spectrometry·Helen J Cooper
Jul 1, 2006·Journal of the American Society for Mass Spectrometry·Corey N W Lam, Ivan K Chu
Aug 8, 2006·Journal of the American Society for Mass Spectrometry·Errol W RobinsonEvan R Williams
Sep 6, 2006·Journal of the American Society for Mass Spectrometry·Haichuan Liu, Kristina Håkansson
Nov 23, 2006·Journal of the American Society for Mass Spectrometry·Jace W JonesFrantisek Turecek
May 25, 2007·Journal of the American Society for Mass Spectrometry·Ryan D LeibEvan R Williams
Oct 2, 2007·Journal of the American Society for Mass Spectrometry·Haichuan Liu, Kristina Håkansson
Apr 11, 2008·Journal of the American Society for Mass Spectrometry·Cheng LinPeter B O'Connor
Mar 25, 2008·Journal of the American Society for Mass Spectrometry·Yury O TsybinAlan G Marshall
Mar 29, 2008·Journal of the American Society for Mass Spectrometry·Jeremy T O'BrienEvan R Williams
Jun 24, 2008·Journal of the American Society for Mass Spectrometry·Kathrin BreukerFred W McLafferty
Oct 22, 2008·Journal of the American Society for Mass Spectrometry·Sarah R HartSimon J Gaskell
Dec 17, 2008·Journal of the American Society for Mass Spectrometry·Shawna M HengelDavid R Goodlett
Dec 30, 2008·Journal of the American Society for Mass Spectrometry·Hisham Ben HamidaneYury O Tsybin
Feb 10, 2009·Journal of the American Society for Mass Spectrometry·Victor A Mikhailov, Helen J Cooper
Mar 4, 2009·Journal of the American Society for Mass Spectrometry·Chi-Kit SiuK W Michael Siu
Mar 20, 2009·Journal of the American Society for Mass Spectrometry·Hisham Ben HamidaneYury O Tsybin
Aug 5, 2009·Journal of the American Society for Mass Spectrometry·Camilla Skinnerup JensenSteen Brøndsted Nielsen
Feb 19, 2010·Journal of the American Society for Mass Spectrometry·Sheng Yin, Joseph A Loo
May 1, 2010·Journal of the American Society for Mass Spectrometry·Qin ZhaoEthan R Badman
Apr 13, 2013·Analytical Chemistry·Jared B ShawJennifer S Brodbelt
May 9, 2013·Chemical Reviews·František Tureček, Ryan R Julian
Mar 31, 2007·Journal of the American Chemical Society·Ryan D LeibEvan R Williams
Apr 19, 2011·Journal of the American Chemical Society·Benjamin N MooreRyan R Julian
Sep 29, 2011·Journal of the American Chemical Society·Hyun Ju YooKristina Håkansson
Sep 3, 2008·Journal of the American Chemical Society·James S PrellEvan R Williams
Apr 1, 2009·Journal of the American Chemical Society·Chang Ho SohnJ L Beauchamp
Oct 7, 2008·The Journal of Physical Chemistry. a·Anne I S HolmEvan R Williams
Nov 27, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kathrin Breuker, Fred W McLafferty
Sep 1, 2009·Bioanalysis·Marcus Macht

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