Nongenetic method for purifying stem cell-derived cardiomyocytes.

Nature Methods
Fumiyuki HattoriKeiichi Fukuda

Abstract

Several applications of pluripotent stem cell (PSC)-derived cardiomyocytes require elimination of undifferentiated cells. A major limitation for cardiomyocyte purification is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamine methyl ester perchlorate, could be used to selectively mark embryonic and neonatal rat cardiomyocytes, as well as mouse, marmoset and human PSC-derived cardiomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardiomyocytes transplanted into testes did not induce teratoma formation. Moreover, aggregate formation of PSC-derived cardiomyocytes through homophilic cell-cell adhesion improved their survival in the immunodeficient mouse heart. Our approaches will aid in the future success of using PSC-derived cardiomyocytes for basic and clinical applications.

References

Jan 30, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kuang Hu, Bazbek Davletov
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kyoko HidakaTakayuki Morisaki
Oct 4, 2003·Blood·Ferdinand H BahlmannDanilo Fliser
Dec 5, 2003·Journal of Molecular and Cellular Cardiology·Arnoud C FijnvandraatAntoon F M Moorman
Jan 14, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jannik N AndersenNiels Peter H Møller
Sep 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Natig GassanovUta C Hoppe
Oct 12, 2005·Biotechnology Annual Review·Monica Monici
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alessandra RecchiaFulvio Mavilio
May 13, 2006·Circulation Research·Mohamed NemirFreddy Radtke
May 23, 2006·Biochemical and Biophysical Research Communications·Tomonori TsukaharaToshikazu Takeshita
Jun 6, 2006·Biochimica Et Biophysica Acta·Stefan Jakobs
Dec 13, 2006·Methods in Enzymology·Michal Gropp, Benjamin Reubinoff
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irit HuberLior Gepstein
Sep 27, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·David AndersonChris Denning

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Citations

Mar 6, 2012·Cellular and Molecular Life Sciences : CMLS·Andre AlconYibing Qyang
Jan 13, 2011·Archives of Toxicology·Anna M Wobus, Peter Löser
Aug 17, 2012·Journal of Cardiovascular Translational Research·Maxime MioulaneSian E Harding
Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hyun-Jai ChoHyo-Soo Kim
Oct 25, 2011·Nature Methods·David A ElliottEdouard G Stanley
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yasunori ShintaniKen Suzuki
Jul 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rie WadaMasaki Ieda
Aug 7, 2010·Antioxidants & Redox Signaling·Mohammad Nurulqadr Jameel, Jianyi Zhang
Nov 5, 2011·Stem Cells and Development·Joshua E BabiarzKyle L Kolaja
Aug 20, 2011·Tissue Engineering. Part C, Methods·Tomohiro HayakawaTeruo Okano
May 25, 2011·Cardiovascular Research·Jan Hendrick van WeerdJun K Takeuchi
May 12, 2012·Stem Cell Research & Therapy·Harold S Bernstein, William C Hyun
Mar 12, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Masaki Ieda
Sep 28, 2010·Regenerative Medicine·Sharon S Y Wong, Harold S Bernstein
Feb 19, 2010·Experimental & Molecular Medicine·Fumiyuki Hattori, Keiichi Fukuda
Dec 1, 2012·Stem Cells Translational Medicine·Yuji HaraguchiTeruo Okano
Feb 23, 2013·Stem Cells Translational Medicine·Pattraranee LimphongIvor Benjamin
Sep 8, 2012·Regenerative Medicine·Harold S Bernstein
Mar 9, 2012·Regenerative Medicine·Brian LiauNenad Bursac
Mar 8, 2013·Future Cardiology·Satsuki FukushimaKen Suzuki
Jan 11, 2014·Stem Cell Research & Therapy·Jean-Sébastien HulotRoger J Hajjar
Oct 2, 2013·BioMed Research International·Nanako KawaguchiToshio Nakanishi
Dec 25, 2013·Stem Cells International·Yohei OhnoKeiichi Fukuda
Mar 1, 2014·Nature Protocols·Uri Ben-David, Nissim Benvenisty
May 20, 2014·Nature Medicine·TingTing HongRobin M Shaw
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hidetaka KiokaSeiji Takashima
Jul 19, 2012·Journal of Cardiovascular Translational Research·Pieterjan DierickxLinda W van Laake
Jun 15, 2014·Current Treatment Options in Cardiovascular Medicine·Sophie Dal-Pra, Maria Mirotsou

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