Noninvasive analyses of polyunsaturated fatty acids in human oral mucosa in vivo by Fourier-transform infrared spectroscopy

Biopolymers
Satoshi Yoshida, Hiroshi Yoshida

Abstract

The aim of this study was to develop a noninvasive method to observe polyunsaturated fatty acids (PUFAs) behavior in the human body using Fourier transform infrared spectroscopy. For the noninvasive measurement of human oral mucosa, we have used infrared spectroscopy with a suitable attachment for an in vivo attenuated total reflectance system. The fatty acid contents in the tissues were determined by gas-chromatography mass-spectrometry after methylation. The alkene C-H stretching vibrations of unsaturated fatty acids in dietary oils showed infrared absorption bands with various peak positions and intensities at around 3010 cm(-1) depending on the extent of unsaturation and their species. The diurnal fluctuation of the alkene peak position of oral mucosa suggested that the contents of PUFAs were increased gradually in the early afternoon, and these data were supported by the direct determination of fatty acid species in oral mucosa where the relative increase of arachidonic and docosahexaenoic acids was observed in the early afternoon. This diurnal change of alkene peak position resembled the pattern of a "lipid factor" change calculated with the factor analysis applied to the overall infrared spectrum. We could monitor the di...Continue Reading

References

Jun 25, 1998·Japanese Journal of Pharmacology·S YoshidaH Okuyama
Feb 23, 1999·The American Journal of Pathology·G A BjarnasonR B Sothern
Oct 8, 1999·Journal of Pediatric Gastroenterology and Nutrition·B KoletzkoA Damli
May 29, 2000·The Proceedings of the Nutrition Society·R UauyC Rojas

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Citations

Dec 26, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna LewinskaMaciej Wnuk
Feb 13, 2007·International Journal of Biological Macromolecules·Vassiliki A IconomidouStavros J Hamodrakas
Apr 13, 2005·Otolaryngologic Clinics of North America·Randall TaggArlen D Meyers
Jan 24, 2007·Archives of Biochemistry and Biophysics·Neslihan ToyranFeride Severcan
May 7, 2015·Forensic Science International : Synergy·Charlotte-Maria OrphanouJohn Cassella
Nov 1, 2016·Journal of the Royal Society, Interface·Gustav WaschatkoSapun H Parekh
May 25, 2006·The Biochemical Journal·Neslihan ToyranFeride Severcan
May 3, 2015·Physiology·Walter ArnoldThomas Ruf
Jun 6, 2015·Journal of Biological Rhythms·Thomas Ruf, Walter Arnold

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