Noninvasive assessment of brain injury in a canine model of hypothermic circulatory arrest using magnetic resonance spectroscopy

The Annals of Thoracic Surgery
Christopher J BarreiroWilliam A Baumgartner

Abstract

Studies have confirmed the neuroprotective effect of diazoxide in canines undergoing hypothermic circulatory arrest (HCA). A decreased N-acetyl-asparate:choline (NAA:Cho) ratio is believed to reflect the severity of neurologic injury. We demonstrated that noninvasive measurement of NAA:Cho with magnetic resonance spectroscopy facilitates assessment of neuronal injury after HCA and allows for evaluation of neuroprotective strategies. Canines underwent 2 hours of HCA at 18 degrees C and were observed for 24 hours. Animals were divided into three groups (n = 15 in each group): normal (unoperated), HCA (HCA only), and HCA+diazoxide (pharmacologic treatment before HCA). The NAA:Cho ratios were obtained 24 hours after HCA by spectroscopy. Brains were immediately harvested for fresh tissue NAA quantification by mass spectrometry. Separate cohorts of HCA (n = 16) and HCA+diazoxide (n = 23) animals were kept alive for 72 hours for daily neurologic assessment. Cortical NAA:Cho ratios were significantly decreased in HCA versus normal animals (1.01 +/- 0.29 versus 1.31 +/- 0.23; p = 0.004), consistent with severe neurologic injury. Diazoxide pretreatment limited neurologic injury versus HCA alone, reflected in a preserved NAA:Cho ratio (1....Continue Reading

References

Dec 20, 1973·The New England Journal of Medicine·J W OlneyT DeGubareff
Nov 1, 1996·The Annals of Thoracic Surgery·M V BrockW A Baumgartner
Aug 14, 1998·Journal of Magnetic Resonance Imaging : JMRI·B D RossC H Shelden
Apr 10, 1999·The Annals of Thoracic Surgery·E E TsengW A Baumgartner
Apr 30, 2003·Anesthesiology·Dara DitsworthC Dean Kurth
Dec 17, 2003·Journal of Neurosurgical Anesthesiology·William A Baumgartner

❮ Previous
Next ❯

Citations

Nov 9, 2007·The New England Journal of Medicine·Michael V Johnston
Oct 29, 2014·American Journal of Veterinary Research·Krystina L StadlerCarl R Jessen
Sep 20, 2011·The Journal of Thoracic and Cardiovascular Surgery·George J ArnaoutakisWilliam A Baumgartner
Aug 26, 2011·Research in Veterinary Science·Paula Martin-VaqueroSteffen Sammet
Nov 11, 2014·Journal of Neuropathology and Experimental Neurology·Mary E BlueJuan C Troncoso
May 13, 2011·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·Charles H Vite, Johnny R Cross
May 13, 2014·Journal of Veterinary Internal Medicine·P J Dickinson
Mar 13, 2009·Research in Veterinary Science·Byeong-Teck KangHee-Myung Park
Dec 10, 2015·Journal of Veterinary Science·Sooyoung ChoiHojung Choi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.