Noninvasive detection of atherosclerotic lesions by 99mTc-based immunoscintigraphic targeting of proliferating smooth muscle cells

Chest
J NarulaB A Khaw

Abstract

Mouse/human chimeric antibody Z2D3 identifies an antigen produced exclusively by proliferating smooth muscle cells of human atheroma, and also cross reacts with experimentally induced atherosclerotic lesions in rabbits. Fab' fragments of Z2D3 antibody were labeled with (99m)Tc using glucaric acid as a weak transchelator. The potential role of (99m)Tc-labeled Z2D3 scintigraphy was explored for noninvasive imaging of experimental atherosclerotic lesions. (99m)Tc-Z2D3 Fab' was utilized for noninvasive imaging in four rabbits with experimentally induced atherosclerotic lesions and in one control rabbit. In addition, (99m)Tc-labeled nonspecific 103D2 Fab' was used for comparison in four other rabbits with atherosclerotic lesions. The atherosclerotic lesions were induced by balloon de-endothelialization of the infradiaphragmatic abdominal aorta and 12 weeks of hyperlipidemic diet. An aliquot of 15 mCi (550 mBq) of (99m)Tc pertechnetate was incubated with 6.25 mg of glucaric acid for 30 min followed by incubation of (99m)Tc glucarate with 375 microg of Z2D3 Fab' or 103D2 Fab' for an additional 30 min. Instant thin-layer chromatography demonstrated almost complete radiolabeling. (99m)Tc-Z2D3 was administered IV and gamma imaging was pe...Continue Reading

Citations

Dec 7, 2000·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·X YuG M Lanza
Dec 18, 2004·European Journal of Nuclear Medicine and Molecular Imaging·Guangming QinShuren Li
Sep 18, 2012·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Dennis H M KustersChris P M Reutelingsperger
Aug 26, 1999·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·A J Fischman
Jul 28, 2012·Therapeutic Delivery·Tatyana S LevchenkoVladimir P Torchilin
May 26, 2004·Cardiology Clinics·Flordeliza S VillanuevaJagat Narula

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