Noninvasive high-resolution in vivo imaging of cell biology in the anterior chamber of the mouse eye.

Nature Protocols
Stephan SpeierPer-Olof Berggren

Abstract

There is clearly a demand for an experimental platform that enables cell biology to be studied in intact vascularized and innervated tissue in vivo. This platform should allow observations of cells noninvasively and longitudinally at single-cell resolution. For this purpose, we use the anterior chamber of the mouse eye in combination with laser scanning microscopy (LSM). Tissue transplanted to the anterior chamber of the eye is rapidly vascularized, innervated and regains function. After transplantation, LSM through the cornea allows repetitive and noninvasive in vivo imaging at cellular resolution. Morphology, vascularization, cell function and cell survival are monitored longitudinally using fluorescent proteins and dyes. We have used this system to study pancreatic islets, but the platform can easily be adapted for studying a variety of tissues and additional biological parameters. Transplantation to the anterior chamber of the eye takes 25 min, and in vivo imaging 1-5 h, depending on the features monitored.

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Citations

May 5, 2012·Nature Protocols·Rayner Rodriguez-DiazAlejandro Caicedo
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Midhat H AbdulredaPer-Olof Berggren
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rayner Rodriguez-DiazAlejandro Caicedo
Oct 21, 2011·Journal of Transplantation·Ping WangAnna Moore
Apr 25, 2013·PloS One·Ryuichi NishimuraMasafumi Goto
Feb 26, 2014·The Journal of Experimental Medicine·Jason MiskaZhibin Chen
Nov 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Erwin IlegemsPer-Olof Berggren
Sep 12, 2013·Molecular Metabolism·Ingo B LeibigerPer-Olof Berggren
Jul 24, 2014·Cellular and Molecular Life Sciences : CMLS·Shao-Nian YangPer-Olof Berggren
Feb 16, 2016·Cell Metabolism·Midhat H AbdulredaPer-Olof Berggren
Feb 24, 2016·Scientific Reports·Meike PaschenPer-Olof Berggren
May 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Karin ÅvallLisa Juntti-Berggren
Feb 9, 2013·Endocrinology and Metabolism Clinics of North America·Rayner Rodriguez-Diaz, Alejandro Caicedo
Oct 2, 2012·Seminars in Cell & Developmental Biology·Alejandro Caicedo
May 10, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Koichi BabaKohji Nishida
May 25, 2010·Biochemical and Biophysical Research Communications·Ingo B LeibigerPer-Olof Berggren
Apr 3, 2010·Trends in Endocrinology and Metabolism : TEM·Daniel EberhardEckhard Lammert
Mar 8, 2016·Islets·Corentin Cras-MéneurErnesto Bernal-Mizrachi
Mar 18, 2016·Biomaterials·Nancy Dekki ShalalyMy Hedhammar
Nov 16, 2013·Immunologic Research·Carmen FotinoAntonello Pileggi
Sep 18, 2013·Diabetes, Obesity & Metabolism·M H Abdulreda, P-O Berggren
May 18, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Y TanV L Perez
Jun 24, 2015·Cell Cycle·Lisa Juntti-BerggrenPer-Olof Berggren
Oct 6, 2014·Advances in Biological Regulation·Christopher J BarkerPer-Olof Berggren
Jul 2, 2014·Journal of Internal Medicine·H Blom, H Brismar
Aug 1, 2015·Nanomedicine·Songeun BeackSei Kwang Hahn
Jan 21, 2015·Experimental Eye Research·Jun Ho LeeKi Hean Kim
Oct 18, 2012·Advances in Biological Regulation·Christopher J BarkerPer-Olof Berggren
Jul 23, 2013·Journal of Autoimmunity·V L PerezF Cruz-Guilloty
Aug 30, 2011·Diabetes·Daniel NyqvistPer-Olof Berggren
Aug 31, 2016·Frontiers in Immunology·Gustaf Christoffersson, Matthias G von Herrath

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