Noninvasive imaging of E-selectin expression by activated endothelium in urate crystal-induced arthritis

Arthritis and Rheumatism
P T ChapmanDorian O Haskard

Abstract

To assess the expression of the cytokine-inducible endothelial leukocyte adhesion molecule E-selectin during the evolution of urate crystal-induced arthritis, using a recently described radiolabeled monoclonal antibody (MAb) imaging technique. Monosodium urate (MSU) crystals and saline alone were injected respectively into the right (inflamed) and left (control) knees of 3 young pigs. Four hours later, 111In-labeled 1.2B6 F(ab')2 (anti-E-selectin MAb) and 125I-labeled MOPC 21 F(ab')2 (control MAb) were injected intravenously. Uptake of 1.2B6 in inflamed and control joints was assessed by scintigraphy 7 and 24 hours after intraarticular injection of MSU crystals. Immunohistochemistry studies and radioactivity counting of tissues were performed postmortem to confirm the observations from scintigraphy. MAb 1.2B6 F(ab')2 scintigraphic images of the knees revealed a significantly increased uptake in the right (inflamed) knee at 7 and 24 hours postinjection, particularly over the joint space. These in vivo images were consistent with E-selectin expression in the inflamed tissue detected by immunohistochemistry and with radioactivity counts postmortem. The synovial localization ratio (inflamed:control synovium counts) was 25.4 +/- 9.7...Continue Reading

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Citations

Nov 13, 2002·Arthritis and Rheumatism·R Clive LandisDorian O Haskard
Jan 25, 2013·Seminars in Immunopathology·Ioannis MitroulisKonstantinos Ritis
Jul 2, 2005·Joint, Bone, Spine : Revue Du Rhumatisme·Ru Liu-Bryan, Frédéric Lioté
Jun 6, 2006·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Mehran M Sadeghi
Aug 28, 2002·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Alberto SignoreFrancesco Scopinaro
Jun 21, 2011·La Presse médicale·Hang-Korng Ea
Jun 9, 1998·The British Journal of Radiology·A M Peters
Sep 29, 2006·Radiology·Peter R ReynoldsA David Edwards
Apr 19, 2015·Arthritis Research & Therapy·Carlos PinedaAlberto López-Reyes
Oct 11, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Emile JubeliGillian Barratt
Jun 1, 1997·Arthritis and Rheumatism·C F Mojcik, E M Shevach
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Julia E ManningHelen M McGettrick

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