Noninvasive Imaging of Islet Transplants with 111In-Exendin-3 SPECT/CT

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Inge van der KroonMartin Gotthardt

Abstract

Islet transplantation is a promising treatment for type 1 diabetic patients. However, there is acute as well as chronic loss of islets after transplantation. A noninvasive imaging method that could monitor islet mass might help to improve transplantation outcomes. In this study, islets were visualized after transplantation in a rat model with a dedicated small-animal SPECT scanner by targeting the glucagonlike peptide-1 receptor (GLP-1R), specifically expressed on β-cells, with (111)In-labeled exendin-3. Targeting of (111)In-exendin-3 to GLP-1R was tested in vitro on isolated islets of WAG/Rij rats. For in vivo evaluation, 400 or 800 islets were transplanted into the calf muscle of WAG/Rij rats (6-8 wk old). Four weeks after transplantation, SPECT/CT images were acquired 1 h after injection of (111)In-labeled exendin-3. After SPECT acquisition, the muscles containing the transplant were analyzed immunohistochemically and autoradiographically. The binding assay, performed on isolated islets, showed a linear correlation between the number of islets and (111)In-exendin-3 accumulation (Pearson r = 0.98). In vivo, a 1.70 ± 0.44-fold difference in tracer uptake between 400 and 800 transplanted islets was observed. Ex vivo analysis of...Continue Reading

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Citations

Dec 26, 2017·Expert Review of Precision Medicine and Drug Development·Genaro A Paredes-JuarezJeff W M Bulte
Aug 5, 2017·Scientific Reports·Wael A EterMartin Gotthardt
Nov 4, 2020·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Hirotake KomatsuYoko Mullen
Feb 17, 2021·PET Clinics·Yaping Luo, Xiaoyuan Chen
Mar 20, 2021·Frontiers in Endocrinology·Dian R Arifin, Jeff W M Bulte
Jun 6, 2018·Bioconjugate Chemistry·Andrej BabičNorbert Lange

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