Noninvasive Molecular Monitoring in Multiple Myeloma Patients Using Cell-Free Tumor DNA: A Pilot Study

The Journal of Molecular Diagnostics : JMD
Giulia BianconPaolo Corradini

Abstract

Novel treatments for multiple myeloma (MM) have increased rates of complete response, raising interest in more accurate methods to evaluate residual disease. Cell-free tumor DNA (cfDNA) analysis may represent a minimally invasive approach complementary to multiparameter flow cytometry (MFC) and molecular methods on bone marrow aspirates. A sequencing approach using the Ion Torrent Personal Genome Machine was applied to identify clonal IGH gene rearrangements in tumor plasma cells (PCs) and in serial plasma samples of 25 patients with MM receiving second-line therapy. The same clonal IGH rearrangement identified in tumor PCs was detected in paired plasma samples, and levels of IGH cfDNA correlated with outcome and mirrored tumor dynamics evaluated using conventional laboratory parameters. In addition, IGH cfDNA levels reflected the number of PCs enumerated by MFC immunophenotyping even in the complete response context. Patients determined by MFC to be free of minimal residual disease were characterized by low frequencies of tumor clonotypes in cfDNA and longer survival. This pilot study supports the clinical applicability of the noninvasive monitoring of tumor levels in plasma samples of patients with MM by IGH sequencing.

Citations

Nov 26, 2019·European Journal of Haematology·David VrabelRoman Hajek
Sep 16, 2020·Journal of Personalized Medicine·Alessandro GozzettiMonica Bocchia
Sep 27, 2019·Frontiers in Oncology·Ilaria Del GiudiceRobin Foà
Mar 18, 2020·Frontiers in Oncology·Niccolo BolliCarolina Terragna
Jul 30, 2020·Clinical Lymphoma, Myeloma & Leukemia·Ioannis Ntanasis-StathopoulosMeletios A Dimopoulos
Mar 3, 2021·Pathology·Jun K LimDipti Talaulikar
Mar 3, 2021·Human Immunology·Lori A Ramkissoon, Nathan D Montgomery
May 1, 2021·Cancers·Miho OgawaArinobu Tojo
Sep 25, 2021·Journal of Hematology & Oncology·Craig T Wallington-Beddoe, Rachel L Mynott
Jul 30, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kenneth C AndersonShaji K Kumar

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