Noninvasive photoacoustic microscopy of methemoglobin in vivo

Journal of Biomedical Optics
Min TangLihong V Wang

Abstract

Due to the various causes of methemoglobinemia and its potential to be confused with other diseases, in vivo measurements of methemoglobin have significant applications in the clinic. Using photoacoustic microscopy (PAM), we quantified the average and the distributed percentage of methemoglobin both in vitro and in vivo. Based on the absorption spectra of methemoglobin, oxyhemoglobin, and deoxyhemoglobin, three wavelengths were chosen to differentiate methemoglobin from the others. The methemoglobin concentrations calculated from the photoacoustic signals agreed well with the preset concentrations. Then we imaged the methemoglobin percentage in microtubes that mimicked blood vessels. Average percentages calculated for five samples with different methemoglobin concentrations also agreed well with the preset values. Finally, we demonstrated the ability of PAM to detect methemoglobin in vivo in a mouse ear. Our results show that PAM can quantitatively image methemoglobin distribution in vivo.

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Citations

Apr 19, 2016·Journal of Biomedical Optics·Yong ZhouLihong V Wang
Jul 28, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jin ZhouHao Wang
Feb 7, 2021·International Journal of Molecular Sciences·Fahima KhatunIzumi Nishidate
Feb 8, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jin ZhouHao Wang

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