Noninvasive Urine Biomarker Lateral Flow Immunoassay for Monitoring Active Onchocerciasis

ACS Infectious Diseases
Ryan J ShireyKim D Janda

Abstract

The parasitic disease onchocerciasis is the second leading cause of preventable blindness, afflicting more than 18 million people worldwide. Despite an available treatment, ivermectin, and control efforts by the World Health Organization, onchocerciasis remains a burden in many regions. With an estimated 120 million people living in areas at risk of infection, efforts are now shifting from prevention to surveillance and elimination. The lack of a robust, point-of-care diagnostic for an active Onchocerca infection has been a limiting factor in these efforts. Previously, we reported the discovery of the biomarker N-acetyl-tyramine- O-glucuronide (NATOG) in human urine samples and its ability to track treatment progression between medicated patients relative to placebo; we also established its capability to monitor disease burden in a jird model. NATOG is a human-produced metabolite of tyramine, which itself is produced as a nematode neurotransmitter. The ability of NATOG to distinguish between active and past infection overcomes the limitations of antibody biomarkers and PCR methodologies. Lateral flow immunoassay (LFIA) diagnostics offer the versatility and simplicity to be employed in the field and are inexpensive enough to be ...Continue Reading

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Citations

Jun 11, 2020·Diagnostics·Emma Michelle Taylor
Jul 25, 2019·Tropical Medicine and Infectious Disease·Jean-Claude Makenga BofYves Coppieters
Jun 16, 2019·Tropical Medicine and Infectious Disease·Jean-Claude Makenga BofYves Coppieters
Jan 26, 2021·ACS Infectious Diseases·Jeffrey D WhitmanMakedonka Mitreva
Jun 5, 2021·Archives of Microbiology·Yachana Gupta, Aditya Sharma Ghrera
Nov 30, 2021·PLoS Neglected Tropical Diseases·Ole LagatieLieven J Stuyver

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Methods Mentioned

BETA
biopsy
enzyme-linked immunosorbent assay
surface plasmon resonance
glycosylation
ELISA
urine composition
ELISAs
chip

Software Mentioned

GraphPad Prism
NATOG

Related Concepts

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