Nonlinear electroosmosis in hierarchical monolithic structures

Electrophoresis
Ivo Nischang, Ulrich Tallarek

Abstract

We studied the dependence of electroosmotic flow (EOF) velocity and separation efficiency for neutral analytes in 100 microm ID capillary monoliths on a variation of the mobile phase ionic strength and applied electrical field strength, i.e., we covered a range for the concentration of Tris buffer from 10(-5) to 10(-2) M and applied electrical field strengths up to 10(5) V/m. The silica-based monoliths are hierarchically structured having intraskeleton mesopores and interskeleton macropores. While a linear dependence of the average EOF velocity on applied field strength could be observed with 5 x 10(-3) M Tris (turning slightly nonlinear at a higher concentration due to thermal effects), this dependence becomes systematically nonlinear as the Tris concentration is reduced towards 10(-4) M. Increased velocities by more than 50% compared to those expected from linear behavior are realized at 10(5) V/m. Concomitantly, as the Tris concentration is reduced from 10(-3) to 10(-4) M, we notice an improvement in plate heights by a factor of more than 2 (they approach 2 microm for ethylbenzoate). We complementary analyzed the onset of the nonlinear EOF dynamics in a hierarchical monolith and the significantly reduced axial dispersion in ...Continue Reading

Citations

Jan 20, 2005·Electrophoresis·Ulrich TallarekIvo Nischang
Feb 14, 2006·Electrophoresis·Myriam Crosnier de BellaistreJean-Louis Rocca
Jul 12, 2007·Journal of Separation Science·Alexandra Höltzel, Ulrich Tallarek
Jan 27, 2007·Electrophoresis·Ivo Nischang, Ulrich Tallarek
Jan 3, 2006·Journal of Chromatography. a·Ivo NischangUlrich Tallarek

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