Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures

Optics Express
Anisha ThayilS Soria

Abstract

We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).

References

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Jan 6, 2008·Sensors·Katrin SchmittChristian Hoffmann

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Citations

Dec 18, 2010·Optics Express·Ori KatzYaron Silberberg
Mar 19, 2020·Micromachines·Gabriele FrigentiSilvia Soria Huguet
Feb 12, 2010·ACS Nano·Maria Teresa MartínezJeffrey Bokor

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