Nonnative helical motif in a chaperone-bound protein fragment

Biophysical Journal
Neşe Kurt, Silvia Cavagnero

Abstract

The effect of cotranslationally active chaperones on the conformation of incomplete protein chains is poorly understood. The secondary structure of a 77-residue chaperone-bound N-terminal protein fragment corresponding to the first five helices (A-E) of apomyoglobin (apoMb(1-77)) is investigated here at the residue-specific level by multidimensional NMR. The substrate-binding domain of DnaK, DnaK-beta, is employed as a chaperone model. By taking advantage of the improved spectral quality resulting from chaperone deuteration, we find that DnaK-beta-bound apoMb(1-77) displays a region of nonnative helicity at residues away from the main chaperone binding site. The nonnative structural motif comprises portions of the native D and E helices and has similar characteristics to the reported nonnative DE helical region of acid-unfolded full-length apoMb. Upon incorporation of the missing C-terminal amino acids, a structural kink develops between residues 56 and 57, and two separate native D and E helices are generated. This work highlights, for the first time to our knowledge, the presence of a nonnative helical motif in a large chaperone-bound protein fragment under physiologically relevant solution conditions.

References

Nov 8, 1996·Journal of Molecular Biology·D Eliezer, P E Wright
Jul 2, 1998·Biophysical Journal·D V Laurents, R L Baldwin
Jan 15, 1999·Journal of Molecular Biology·J L Neira, A R Fersht
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Shang-Te Danny HsuJohn Christodoulou

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Citations

May 10, 2011·Journal of the American Chemical Society·Jung Ho LeeSilvia Cavagnero
Jul 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jung Ho LeeSilvia Cavagnero
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ashok SekharLewis E Kay
May 3, 2012·Protein Science : a Publication of the Protein Society·Ashok SekharSilvia Cavagnero
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Ashok SekharLewis E Kay
Jun 16, 2019·The Biochemical Journal·Eugenia M ClericoLila M Gierasch
Mar 23, 2019·Annual Review of Biophysics·Ashok Sekhar, Lewis E Kay
Jan 22, 2013·Biochemistry·Satyam TiwariKoyeli Mapa

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