PMID: 9171077Jun 15, 1997Paper

NonO enhances the association of many DNA-binding proteins to their targets

Nucleic Acids Research
Y S YangJ D Capra

Abstract

NonO is an unusual nucleic acid binding protein not only in that it binds both DNA and RNA but that it does so via functionally separable domains. Here we document that NonO enhances the binding of some (E47, OTF-1 and OTF-2) but not all (PEA3) conventional sequence-specific transcription factors to their recognition sites in artificial substrates as well as in an immunoglobulin VHpromoter. We also show that NonO induces the binding of the Ku complex to DNA ends. Ku has no known DNA sequence specificity. These enhancement of binding effects are NonO concentration dependent. Using the E box activity of E47 as a model, kinetic studies demonstrate that the association rate of the protein-DNA complex increases in the presence of NonO while the dissociation rate remains the same, thereby increasing the sum total of the interaction. Oligo competition experiments indicate that NonO does not contact the target DNA in order to enhance the binding activity of DNA binding proteins. Rather, methylation interference analysis reveals that the induced E47 binding-activity has the same DNA-binding sequence specificity as the normal binding. This result suggests that one of the effects of NonO is to induce a true protein-DNA interaction. In thi...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A Voronova, D Baltimore
Jul 30, 1990·Biochimica Et Biophysica Acta·M BustinD Landsman
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·W Du, T Maniatis
Apr 25, 1993·Nucleic Acids Research·Y S YangJ D Capra
Mar 1, 1993·Genes & Development·J G PattonB Nadal-Ginard
Jun 1, 1993·Molecular and Cellular Biology·G BainC Murre

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Citations

Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg
Nov 22, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Daniel M PassonCharles S Bond
Aug 9, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Céline BruelleMichel Vincent
May 5, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Cheng ZhangXing Li Wang
Dec 18, 2013·Human Molecular Genetics·Tatyana A ShelkovnikovaVladimir L Buchman
Sep 2, 2009·The Journal of Cell Biology·Charles S Bond, Archa H Fox
Nov 6, 2002·FEBS Letters·Yaron Shav-Tal, Dov Zipori
Jul 1, 2010·Wiley Interdisciplinary Reviews. RNA·Ling-Ling Chen, Gordon G Carmichael
Mar 15, 2015·AIDS Research and Human Retroviruses·Corine St GelaisLi Wu
Nov 15, 2008·Molecular and Cellular Endocrinology·Marion B Sewer, Srinath Jagarlapudi
Jan 16, 2002·Current Biology : CB·Archa H FoxAngus I Lamond
Sep 30, 2016·Scientific Reports·Tsui-Ting HoYin-Yuan Mo
Jun 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Maria Girbes MinguezRalf Kleene
May 24, 2000·The Journal of Biological Chemistry·P KarhumaaH Rajaniemi
Sep 15, 2011·PloS One·Shufen Angeline OngKen-Shiung Chen
Jan 15, 2021·Scientific Reports·Mansi SrivastavaSarath Chandra Janga

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