Nonopsonic binding of Mycobacterium tuberculosis to human complement receptor type 3 expressed in Chinese hamster ovary cells.

Infection and Immunity
C CywesM R Ehlers

Abstract

Nonopsonic invasion of mononuclear phagocytes by Mycobacterium tuberculosis is likely important in the establishment of a primary infection in the lung. M. tuberculosis binds to a variety of phagocyte receptors, of which the mannose receptor and complement receptor type 3 (CR3) may support nonopsonic binding. CR3, a beta2 integrin, is a target for diverse intracellular pathogens, but its role in nonopsonic binding remains uncertain. We have examined the binding of M. tuberculosis H37Rv to human CR3 heterologously expressed in Chinese hamster ovary (CHO) cells, thereby circumventing the problems of competing receptors and endogenously synthesized complement, which are inherent in studies with mononuclear phagocytes. The surface expression of CD11b and CD18 was assessed by immunofluorescence, immunobead binding, flow cytometry, and immunoprecipitation with anti-CD11b and anti-CD18 monoclonal antibodies (MAbs). The functional activity of the surface-expressed CD11b/CD18 (CR3) heterodimer was confirmed by rosetting with C3bi-coated microspheres. We found that M. tuberculosis bound four- to fivefold more avidly to CR3-expressing CHO cells than to wild-type cells and, importantly, that this binding was at similar levels in the presen...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·S FalkowD A Portnoy
Aug 21, 1992·Science·B R Bloom, C J Murray
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M R EhlersJ F Riordan
Apr 1, 1990·Immunological Reviews·R S Larson, T A Springer
Jul 1, 1985·The Journal of Experimental Medicine·J M BlackwellS Gordon
Dec 1, 1983·The Journal of Experimental Medicine·S D Wright, S C Silverstein
Mar 1, 1994·The Journal of Cell Biology·T E O'TooleM H Ginsberg
Apr 1, 1995·The Journal of Experimental Medicine·R R Ingalls, D T Golenbock
Sep 1, 1995·The Journal of Cell Biology·M S DiamondT A Springer
Jan 1, 1995·Annual Review of Biochemistry·P J Brennan, H Nikaido
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·J F DowningW J Martin
Jan 1, 1994·Trends in Microbiology·R R Isberg, G Tran Van Nhieu
Jul 1, 1993·Infection and Immunity·M K HondalusD M Mosser
May 1, 1996·Immunology Today·H R Petty, R F Todd

❮ Previous
Next ❯

Citations

Oct 23, 1997·Nature Medicine·Y Zaffran, J J Ellner
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C HuP Salgame
Nov 14, 2001·Infection and Immunity·S L Mueller-OrtizS J Norris
Nov 11, 1999·Clinical and Experimental Immunology·J FäldtM Ridell
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Aug 8, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Véronique Le CabecIsabelle Maridonneau-Parini
Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhi-Hong XueSuet-Mien Tan
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·P PeyronI Maridonneau-Parini
Nov 5, 2011·The Journal of Biological Chemistry·Geok-Lin ChuaSurajit Bhattacharjya
Sep 15, 2020·Frontiers in Cellular and Infection Microbiology·Jacques AugenstreichCatherine Astarie-Dequeker
Jan 28, 2009·The Journal of Cell Biology·Elsa-Noah N'DiayeWilliam E Seaman
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Isabelle VergneVojo Deretic
Jul 20, 2019·The Biochemical Journal·Rainer KalscheuerRafael Prados-Rosales
Apr 7, 1998·Infection and Immunity·J D Ernst
Jul 18, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M D Melo, R W Stokes
Feb 3, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M Daffé, G Etienne
Apr 1, 1998·Clinical and Diagnostic Laboratory Immunology·G HetlandM Harboe
Aug 21, 2003·Microbial Pathogenesis·Marco Antonio Velasco-VelázquezJosé Agramonte-Hevia
Jul 14, 2007·Veterinary Immunology and Immunopathology·Paulraj K Lawrence, Subramaniam Srikumaran
Aug 25, 2009·Molecular Immunology·Maria V CarrollRobert B Sim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.