Nonoxidative metabolism of 2-butoxyethanol via fatty acid conjugation in Fischer 344 rats

Journal of Toxicology and Environmental Health
B S KaphaliaG A Ansari

Abstract

Nonoxidative metabolism of ethylene glycol monobutyl ether (2-butoxyethanol or BE) via fatty acid conjugation was studied in the liver of Fischer 344 male rats following a single oral administration of 500 mg/kg body weight [ethyl-1,2-14C]BE (70 microCi/kg). Animals were killed 2 h after the treatment, hepatic lipids extracted, and the neutral lipids were separated using solid-phase extraction. The neutral lipid fraction was subjected to preparative thin-layer chromatography, and the esters corresponding to the relative flow of authentic fatty acid 2-butoxyethyl esters were recovered and analyzed by reversed-phase high-performance liquid chromatography (HPLC) using methanol-water (37:3, v/v) as solvent. Approximately 85% of the 14C label present in the ester fraction was coeluted at retention times corresponding to the different fatty acid 2-butoxyethyl ester standards. The radioactive fractions were analyzed by electron impact mass spectrometry. Molecular ion peaks and fragmentation patterns similar to that of 16:0, 18:0, 18:1, 18:2, and 20:4 fatty acid 2-butoxyethyl ester standards were detected in the corresponding radioactive HPLC fractions. Fatty acid ethyl ester synthase (FAEES), purified from the rat liver microsomal fra...Continue Reading

Citations

May 20, 1999·Annual Review of Pharmacology and Toxicology·R C Baker, R E Kramer
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
May 2, 2020·Biodegradation·Christine WoiskiKarl-Heinrich Engesser

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