PMID: 6407757Jun 1, 1983Paper

Nonpackaging and packaging proteins of hnRNA in Drosophila melanogaster

Cell
W RisauM Frasch

Abstract

Monoclonal antibodies have previously been raised against chromosomal proteins of Drosophila. Using a biochemical fractionation method for the isolation of large hnRNA-containing structures (hnRNP) of Drosophila tissue culture cells, we show that seven of these antibodies recognize different antigens, and that these antigens are associated with RNA. Analysis of the sedimentation behavior of antigen-containing structures in sucrose gradients reveals that the antigens are differentially distributed with respect both to one another and to pulse-labeled RNA. We demonstrate that the antigens are minor components of hnRNP and are different from the major Drosophila hnRNP packaging proteins, which we have also identified. The antigens are probably involved in the processing of hnRNA in the nucleus.

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Citations

Jan 1, 1986·Journal of Molecular Evolution·E Zuckerkandl
May 11, 2011·Chromosoma·Juliet L RedhouseRobert A H White
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T J HulsebosW Hennig
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·Y D Choi, G Dreyfuss
Jan 1, 1992·The Journal of Cell Biology·M J MatunisG Dreyfuss
Apr 1, 1993·The Journal of Cell Biology·E L MatunisG Dreyfuss
Aug 19, 2007·BMC Cell Biology·Roberto Piergentili
Apr 15, 2008·BMC Genetics·Francesca CepraniRoberto Piergentili
Jun 6, 2006·Experimental Cell Research·Natalia KozlovaNeus Visa
Nov 19, 2004·International Review of Cytology·I F ZhimulevE N Andreyeva
Apr 24, 2008·Journal of Cell Science·Roberto Piergentili, Caterina Mencarelli
Jul 1, 1994·Molecular and Cellular Biology·M E SamuelsP Schedl
Feb 1, 1992·Molecular and Cellular Biology·G RaychaudhuriA L Beyer

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