Nonpathological extracellular amyloid is present during normal epididymal sperm maturation.

PloS One
Sandra WhellyG A Cornwall

Abstract

Amyloids are aggregated proteins characterized by a specific cross-β-sheet structure and are typically associated with neurodegenerative diseases including Alzheimer's disease. Recently, however, several nonpathological amyloids have been found in intracellular organelles of normal mammalian tissues suggesting that amyloid may also carry out biological functions. We previously have shown that the epididymal cystatin CRES (cystatin-related epididymal spermatogenic), cst8, a reproductive-specific member of the cystatin superfamily of cysteine protease inhibitors, forms amyloid in vitro suggesting that CRES amyloid may also form in vivo within the epididymal lumen. Here we show that amyloid structures containing CRES are a component of the normal mouse epididymal lumen without any apparent cytotoxic effects on spermatozoa and that these structures change along the length of the tubule. These studies suggest the presence of a functional amyloid structure that may carry out roles in sperm maturation or maintenance of the luminal milieu and which itself may undergo maturational changes along the epididymis. In contrast to previous examples of functional amyloid which were intracellular, our studies now show that nonpathological/funct...Continue Reading

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Citations

Jun 19, 2012·Molecular & Cellular Proteomics : MCP·Benoit GuyonnetGail A Cornwall
Mar 18, 2016·Cellular and Molecular Neurobiology·Guillaume van Niel
Sep 2, 2014·Molecular and Biochemical Parasitology·Amornrat GeadkaewRudi Grams
Aug 19, 2014·Essays in Biochemistry·Chi L L PhamMargaret Sunde
May 17, 2014·Prion·Raimon Sabate
Jun 5, 2015·PloS One·Nathan EggeGail A Cornwall
Sep 10, 2016·PloS One·Ganapathy Narmadha, Suresh Yenugu
Jul 1, 2017·Biomolecules·Aveline HewetsonGail A Cornwall
May 7, 2014·Molecular and Cellular Biology·Benoit GuyonnetGail A Cornwall
Dec 28, 2012·Biology of Reproduction·Marvin FerrerRichard Oko
May 16, 2019·Cold Spring Harbor Perspectives in Biology·Daniel Otzen, Roland Riek
Feb 7, 2014·The Journal of Biological Chemistry·Sandra WhellyGail A Cornwall
Feb 13, 2018·Biomaterials Science·Chen LiPeng Yang
Jul 25, 2019·Journal of Assisted Reproduction and Genetics·Ricardo N PimentelDavid Lawrence Keefe
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Aveline HewetsonGail A Cornwall
Sep 25, 2017·Biomolecules·Matthew P Jackson, Eric W Hewitt
Sep 13, 2019·FEBS Letters·Dane MarijanTimothy E Audas
Feb 26, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kristina Endres
Aug 14, 2020·International Journal of Molecular Sciences·Vince SzegeczkiTamás Juhász
Jan 22, 2019·Andrology·G A CornwallC Myers
Jun 28, 2017·ELife·Nadia R RoanWarner C Greene
Oct 28, 2020·The Journal of Biological Chemistry·Daniel L MorrisNico Tjandra
Dec 16, 2020·Microorganisms·Anthony BalistreriMatthew Chapman
Jan 12, 2021·The Journal of Biological Chemistry·Hoa Quynh DoGail A Cornwall
Jan 18, 2021·The Journal of Biological Chemistry·Daniel L MorrisNico Tjandra
Mar 17, 2021·Asian Journal of Andrology·Annika SonessonAndreas Hillarp
May 4, 2021·Frontiers in Cell and Developmental Biology·Shenae L CafeElizabeth G Bromfield

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Methods Mentioned

BETA
gene knockout
filter
dot blot
X-ray
Transmission electron microscopy
fluorescence microscopy
electron microscopy
pulldown

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