Nonradioactive sequence-tagged microsatellite site analyses: a method transferable to the tropics

Electrophoresis
P J LagodaJ L Noyer

Abstract

Utilization of existing isozyme analysis facilities to detect sequence-tagged microsatellite site (STMS) polymorphism or any simple sequence repeat (SSR) variation is described. Different parameters concerning the difficulties in transferring molecular techniques to less sophisticated laboratory infrastructures (i.e. tropical outstations) are discussed (e.g. reproducibility, efficacy, precision). Nonradioactive STMS analysis is bound to foster collaborative research between "biodiversity" and "biotechnology" centers.

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Citations

Jul 1, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bradley J TillChikelu Mba
Oct 21, 2015·Genomics Data·Manu KumarAshwani Pareek
Aug 8, 2015·PloS One·Jana ČížkováJaroslav Doležel

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