Nonregeneration protocol for surface plasmon resonance: study of high-affinity interaction with high-density biosensors

Analytical Chemistry
Yijun TangXiangqun Zeng

Abstract

Surface plasmon resonance (SPR) has been used in determining kinetics and thermodynamics of biological interaction in the past decades. One difficulty encountered in this technology is the need for a proper regeneration, which means the removal of analytes from the bound complexes to regenerate the activity of the ligands. Regeneration is not always practical since the harsh regeneration reagents may destroy the bioactivity of the ligands. It is even more difficult for complexes with high affinity constants. In this paper, we report a nonregeneration protocol for SPR techniques in which subsequent ligand/analyte interactions can be measured without regeneration; thus ligand biological activity could be retained. Kinetics, binding models, and mathematics of this protocol are discussed in detail using rabbit IgG as the analyte and engineered recombinant antibody A10B single-chain fragment variables (scFv) as the ligand. The affinity constant of rabbit IgG binding with A10B scFv measured by using a nonregeneration protocol was (2.5 +/- 0.2) x 10(7) M(-1), which was comparable with the value determined with a conventional regeneration SPR method ((2.2 +/- 1.5) x 10(7) M(-1)) and quartz crystal microbalance (1.9 x 10(7) M(-1)). A pa...Continue Reading

References

Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W A HendricksonR P Phizackerley
Nov 5, 1993·Journal of Immunological Methods·R KarlssonB Persson
Jun 20, 1996·Chemical Reviews·Abraham Ulman
Apr 23, 2002·Protein Science : a Publication of the Protein Society·Yasmina S N DayDavid G Myszka
May 12, 2004·Journal of Molecular Recognition : JMR·Robert Karlsson
Feb 1, 2005·Analytical Chemistry·Zhihong ShenXiangqun Zeng

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Citations

May 8, 2010·Analytical and Bioanalytical Chemistry·Fernando GarayErno Lindner
Aug 29, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Huamin LiangMarjo Yliperttula
Feb 15, 2007·Analytical Chemistry·Zhihong ShenXiangqun Zeng
Apr 11, 2009·Analytical Biochemistry·Jing YuanYinqiu Wu
Jun 9, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Pedro M R PauloSílvia M B Costa
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Tianyu WangWei Liu
Jul 14, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Linling BaiChen Wang
Aug 1, 2018·Nature Communications·Nadia LeloupBert J C Janssen
Feb 18, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Amir SyahirKotaro Kajikawa
Feb 21, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Amir SyahirHisakazu Mihara
Mar 25, 2011·Nano Letters·Si ChenMikael Käll
Sep 7, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Xiang-Lin MengZhi-Kang Xu
Jul 3, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Hirokazu SetoYoshiko Miura

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